Femtoliter Scale Detection of an Antithyroid Drug Using Gold Nanoparticles in a Microfluidic Chip
| Autor: | Nader Shokoufi, Kazem Kargosha, Sara Adeleh, Atefeh Abbasi-Ahd |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Detection limit
Materials science 010401 analytical chemistry Microfluidics Analytical chemistry Femtoliter Nanotechnology 02 engineering and technology General Chemistry 021001 nanoscience & nanotechnology glass microfluidic chip 01 natural sciences 0104 chemical sciences photo thermal lens microscopy Methimazole Microfluidic chip Colloidal gold medicine Surface plasmon resonance 0210 nano-technology Absorption (electromagnetic radiation) methimazole gold nanoparticle surface plasmon resonance medicine.drug |
| Zdroj: | Journal of the Brazilian Chemical Society v.28 n.10 2017 Journal of the Brazilian Chemical Society Sociedade Brasileira de Química (SBQ) instacron:SBQ Journal of the Brazilian Chemical Society, Volume: 28, Issue: 10, Pages: 1843-1849, Published: OCT 2017 |
| Popis: | Sensitive detection of methimazole (MMI) at the femtoliter (fL) scale has been achieved based on photo thermal lensing of gold nanoparticles (GNPs). GNPs were used because their surface plasmon resonance (SPR) have absorption in visible range. A combined glass microfluidic chip-photo thermal lens microscopy (MFC-PTLM) system with appropriate laser source was assembled for detection of MMI with GNPs. The thiol group of MMI bound to the surface of GNPs and created the core-shell form and reduced the surface plasmon resonance of GNPs as a consequence the PTLM signal of GNPs decreased following the increase of MMI concentration. The results showed that the changes of PTLM signal in the focal volume of 2.68 fL were proportional to the concentration of MMI over the range of 75-900 × 10-9 mol L-1. The obtained detection limit of this method was 46.5 × 10-9 mol L-1 and the relative standard deviation for ten repeated measurements of 550 × 10-9 mol L-1 MMI solutions was 3.47%. The developed method was successfully applied for determination of MMI in human serum and pharmaceutical samples. |
| Databáze: | OpenAIRE |
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