What is (Still not) Known of the Mechanism by Which Electroporation Mediates Gene Transfer and Expression in Cells and Tissues

Autor: Marie-Pierre Rols, Justin Teissié, Jean-Michel Escoffre, Luc Wasungu, Thomas Portet, David S. Dean
Přispěvatelé: Institut de pharmacologie et de biologie structurale (IPBS), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Physique Statistique des Systèmes Complexes (LPT) (PhyStat), Laboratoire de Physique Théorique (LPT), Institut de Recherche sur les Systèmes Atomiques et Moléculaires Complexes (IRSAMC), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche sur les Systèmes Atomiques et Moléculaires Complexes (IRSAMC), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Escoffre, Jean-Michel
Rok vydání: 2008
Předmět:
Cell Membrane Permeability
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
[SDV.BA] Life Sciences [q-bio]/Animal biology
Electrochemotherapy
[SDV]Life Sciences [q-bio]
Cell
Gene Expression
Bioengineering
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Biology
Applied Microbiology and Biotechnology
Biochemistry
[PHYS.COND.CM-SM] Physics [physics]/Condensed Matter [cond-mat]/Statistical Mechanics [cond-mat.stat-mech]
Mice
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
medicine
Animals
Humans
[PHYS.COND.CM-SM]Physics [physics]/Condensed Matter [cond-mat]/Statistical Mechanics [cond-mat.stat-mech]
[SDV.BC] Life Sciences [q-bio]/Cellular Biology
Molecular Biology
Gene
030304 developmental biology
0303 health sciences
[SDV.BA]Life Sciences [q-bio]/Animal biology
Electroporation
Genetic transfer
Gene Transfer Techniques
Small molecule
[SDV.BIO] Life Sciences [q-bio]/Biotechnology
Cell biology
[SDV] Life Sciences [q-bio]
medicine.anatomical_structure
Membrane
chemistry
Cytoplasm
030220 oncology & carcinogenesis
DNA
Plasmids
Biotechnology
Zdroj: Molecular Biotechnology
Molecular Biotechnology, Humana Press, 2009, 41 (3), pp.286-295. ⟨10.1007/s12033-008-9121-0⟩
Molecular Biotechnology, 2009, 41 (3), pp.286-295. ⟨10.1007/s12033-008-9121-0⟩
ISSN: 1559-0305
1073-6085
DOI: 10.1007/s12033-008-9121-0
Popis: International audience; Cell membranes can be transiently permeabilized under application of electric pulses. This treatment allows hydrophilic therapeutic molecules, such as anticancer drugs and DNA, to enter into cells and tissues. This process, called electropermeabilization or electroporation, has been rapidly developed over the last decade to deliver genes to tissues and organs, but there is a general agreement that very little is known about what is really occurring during membrane electropermeabilization. It is well accepted that the entry of small molecules, such as anticancer drugs, occurs mostly through simple diffusion after the pulse while the entry of macromolecules, such as DNA, occurs through a multistep mechanism involving the electrophoretically driven interaction of the DNA molecule with the destabilized membrane during the pulse and then its passage across the membrane. Therefore, successful DNA electrotransfer into cells depends not only on cell permeabilization but also on the way plasmid DNA interacts with the plasma membrane and, once into the cytoplasm, migrates towards the nucleus. The focus of this review is to describe the different aspects of what is known of the mechanism of membrane permeabilization and associated gene transfer and, by doing so, what are the actual limits of the DNA delivery into cells.
Databáze: OpenAIRE
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