Osteogenesis induced in goat bone marrow progenitor cells by recombinant adenovirus coexpressing bone morphogenetic protein 2 and basic fibroblast growth factor

Autor: Shouting Liu, Qi-Cai Liu, Xufang Li, Qi-Feng Guo, Hui-Hua Xu
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Physiology
Cellular differentiation
Basic fibroblast growth factor
Bone Morphogenetic Protein 2
medicine.disease_cause
T2A sequence
Biochemistry
chemistry.chemical_compound
Osteogenesis
Osteogenic differentiation
Medicine
Adenovirus
General Pharmacology
Toxicology and Pharmaceutics
lcsh:QH301-705.5
lcsh:R5-920
General Neuroscience
Goats
Stem Cells
Gene Transfer Techniques
Cell Differentiation
General Medicine
Immunohistochemistry
Recombinant Proteins
embryonic structures
cardiovascular system
Alkaline phosphatase
Fibroblast Growth Factor 2
Stem cell
lcsh:Medicine (General)
animal structures
Immunology
Genetic Vectors
Primary Cell Culture
Biophysics
BMP2
Bone Marrow Cells
Enzyme-Linked Immunosorbent Assay
Bone healing
Bone morphogenetic protein 2
Adenoviridae
Centrifugation
Density Gradient
Animals
Clinical Investigation
Analysis of Variance
Osteoblasts
Base Sequence
business.industry
fungi
Cell Biology
Alkaline Phosphatase
Molecular biology
biological factors
chemistry
bFGF
lcsh:Biology (General)
Cell culture
BMPCs
business
Zdroj: Brazilian Journal of Medical and Biological Research, Vol 46, Iss 9, Pp 809-814 (2013)
Brazilian Journal of Medical and Biological Research
Brazilian Journal of Medical and Biological Research v.46 n.9 2013
Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
Brazilian Journal of Medical and Biological Research, Volume: 46, Issue: 9, Pages: 809-814, Published: 06 SEP 2013
Popis: Bone morphogenetic protein 2 (BMP2) and basic fibroblast growth factor (bFGF) have been shown to exhibit a synergistic effect to promote bone repair and healing. In this study, we constructed a novel adenovirus with high coexpression of BMP2 and bFGF and evaluated its effect on osteogenic differentiation of goat bone marrow progenitor cells (BMPCs). Recombinant adenovirus Ad-BMP2-bFGF was constructed by using the T2A sequence. BMPCs were isolated from goats by density gradient centrifugation and adherent cell culture, and were then infected with Ad-BMP2-bFGF or Ad-BMP2. Expression of BMP2 and bFGF was detected by ELISA, and alkaline phosphatase (ALP) activity was detected by an ALP assay kit. In addition, von Kossa staining and immunocytochemical staining of collagen II were performed on BMPCs 21 days after infection. There was a high coexpression of BMP2 and bFGF in BMPCs infected with Ad-BMP2-bFGF. Twenty-one days after infection, ALP activity was significantly higher in BMPCs infected with Ad-BMP2-bFGF than in those infected with Ad-BMP2. Larger and more mineralized calcium nodules, as well as stronger collagen II staining, were observed in BMPCs infected with Ad-BMP2-bFGF than in those infected with Ad-BMP2. In summary, we developed a novel adenovirus vector Ad-BMP2-bFGF for simultaneous high coexpression of BMP2 and bFGF, which could induce BMPCs to differentiate efficiently into osteoblasts.
Databáze: OpenAIRE
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