Human Monocytes/Macrophages Release TNF-α in Response to Ox-LDL
| Autor: | Jan Nilsson, Bengt Kallin, Stefan Jovinge, Mikko P.S. Ares |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Stimulation Biology Monocytes Muscle Smooth Vascular chemistry.chemical_compound Internal medicine medicine Humans Macrophage Secretion Cells Cultured Tumor Necrosis Factor-alpha Cell growth Macrophages Monocyte Growth factor Lipoproteins LDL medicine.anatomical_structure Endocrinology chemistry Low-density lipoprotein Tumor necrosis factor alpha Cardiology and Cardiovascular Medicine Cell Division |
| Zdroj: | Arteriosclerosis, Thrombosis, and Vascular Biology. 16:1573-1579 |
| ISSN: | 1524-4636 1079-5642 |
| DOI: | 10.1161/01.atv.16.12.1573 |
| Popis: | The uptake of oxidatively modified low density lipoprotein (Ox-LDL) by intimal macrophages is believed to play a key role in the development of atherosclerosis. The present study demonstrates that Ox-LDL in low concentrations activates monocyte/macrophage release of factors that stimulate smooth muscle cell growth, whereas higher concentrations are inhibitory. Exposure of monocytes/macrophages to 8 μg/mL Ox-LDL increased expression of tumor necrosis factor-α (TNF-α) mRNA but had no effect on interleukin-1β, platelet-derived growth factor B and heparin-binding epidermal growth factor–like mitogen mRNA levels. Ox-LDL also stimulated monocyte/macrophage release of TNF-α in a dose-dependent manner, with maximal effect at an LDL concentration of 8 μg/mL. Addition of TNF-α–blocking antibodies to conditioned medium from monocytes/macrophages already exposed to Ox-LDL reduced mitogenic activity by 44.7±8.4% ( P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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