Detection of Autoantibodies to Vascular Endothelial Growth Factor Receptor-3 in Bile Duct Ligated Rats and Correlations with a Panel of Traditional Markers of Liver Diseases

Autor: Fernando Ponz, Delia E. Cruz-Vega, Flora Sánchez, Jorge E. Moreno-Cuevas, Gabriela Alarcón-Galván, Florent Duval, María Teresa González-Garza, Ivonne González-Gamboa
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Male
Pathology
Clinical Biochemistry
Wistar rat
immunology
chemistry.chemical_compound
Liver disease
disease marker
0302 clinical medicine
antibody detection
Fibrosis
vasculotropin receptor 3
rat
animal
liver fibrosis
Liver injury
bilirubin blood level
lcsh:R5-920
Cholestasis
Bile duct
Liver Diseases
fibroblast growth factor receptor 3
antibody blood level
General Medicine
Vascular endothelial growth factor
medicine.anatomical_structure
priority journal
030211 gastroenterology & hepatology
lcsh:Medicine (General)
liver disease
Research Article
liver injury
medicine.medical_specialty
Article Subject
Bilirubin
bile duct ligation
animal experiment
Article
animal tissue
03 medical and health sciences
Antigens
Neoplasm
Genetics
medicine
Animals
Humans
Receptor
Fibroblast Growth Factor
Type 3
controlled study
human
Rats
Wistar
Molecular Biology
Autoantibodies
nonhuman
business.industry
aminotransferase
disease model
Biochemistry (medical)
Autoantibody
medicine.disease
enzyme linked immunosorbent assay
Rats
Disease Models
Animal
030104 developmental biology
chemistry
Fgfr3 protein
mouse
7 INGENIERÍA Y TECNOLOGÍA
business
metabolism
autoantibody
tumor antigen
Zdroj: Repositorio de Resultados de Investigación del INIA
INIA: Repositorio de Resultados de Investigación del INIA
Disease Markers
Disease Markers, Vol 2016 (2016)
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA
ISSN: 0278-0240
DOI: 10.1155/2016/6597970
Popis: There is a need for new noninvasive biomarkers (NIBMs) able to assess cholestasis and fibrosis in chronic cholestatic liver diseases (CCLDs). Tumorigenesis can arise from CCLDs. Therefore, autoantibodies to tumor-associated antigens (TAA) may be early produced in response to abnormal self-antigen expression caused by cholestatic injury. Vascular endothelial growth factor receptor-3 (VEGFR-3) has TAA potential since it is involved in cholangiocytes and lymphatic vessels proliferations during CCLDs. This study aims to detect autoantibodies directed at VEGFR-3 during bile duct ligation- (BDL-) induced cholestatic injury in rat sera and investigate whether they could be associated with traditional markers of liver damage, cholestasis, and fibrosis. An ELISA was performed to detect anti-VEGFR-3 autoantibodies in sera of rats with different degree of liver injury and results were correlated with aminotransferases, total bilirubin, and the relative fibrotic area. Mean absorbances of anti-VEGFR-3 autoantibodies were significantly increased from week one to week five after BDL. The highest correlation was observed with total bilirubin (R2= 0.8450,P=3.04e-12). In conclusion, anti-VEGFR-3 autoantibodies are early produced during BDL-induced cholestatic injury, and they are closely related to cholestasis, suggesting the potential of anti-VEGFR-3 autoantibodies as NIBMs of cholestasis in CCLDs and justifying the need for further investigations in patients with CCLD.
Databáze: OpenAIRE
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