Molecular Characterization of BK and JC Viruses Circulating among Potential Kidney Donors in Kuwait
| Autor: | Wassim Chehadeh, Mangalathillam Raman Nampoory, Susan Silpi Kurien |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Article Subject viruses JC virus lcsh:Medicine Urine Biology medicine.disease_cause General Biochemistry Genetics and Molecular Biology Nephropathy Genotype medicine Humans Kidney transplantation Aged Kidney Base Sequence General Immunology and Microbiology lcsh:R General Medicine Middle Aged medicine.disease JC Virus Kidney Transplantation Virology Tissue Donors BK virus Transplantation medicine.anatomical_structure Kuwait BK Virus DNA Viral Clinical Study Female |
| Zdroj: | BioMed Research International, Vol 2013 (2013) BioMed Research International |
| ISSN: | 2314-6133 |
| DOI: | 10.1155/2013/683464 |
| Popis: | BK and JC polyomaviruses can be associated with nephropathy following renal transplantation. The aim of this study was to determine the prevalence, load, and genotypes of BK and JC viruses circulated in potential kidney donors in Kuwait. The detection of polyomavirus DNA was carried out in serum and urine samples of 165 potential kidney donors. Seventy (42%) individuals were tested positive for polyomavirus DNA, of whom 20 (12%) had detectable polyomavirus DNA in their serum samples, 40 (24%) in their urine samples, and 10 (6%) in both serum and urine samples. In the group of polyomavirus-positive patients, JC DNA could be detected in 78% of urine samples and 11% of serum samples, whereas BK DNA could be detected in 7% of urine samples and 3% of serum samples. The median polyomaviral load was low. The detected BK sequences in Kuwaiti adults formed new clusters sharing common ancestor with subgroups Ib1 and IVc, which are prevalent in Asia and Europe. Additionally, around half of the detected JCV sequences in Kuwaiti adults formed new clusters within the African subtype 3. Our results suggest high rate of polyomavirus shedding among healthy adults in Kuwait that can jeopardize their suitability for kidney donation. |
| Databáze: | OpenAIRE |
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