Nitric oxide synthase activity in brain tissues from llama fetuses submitted to hypoxemia
| Autor: | Emilia M. Sanhueza, Gina Sánchez, M. Antonieta Valenzuela, Aníbal J. Llanos, Raquel A. Riquelme, M. Galleguillos, Jorge P. Figueroa |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
medicine.medical_specialty
Physiology Blotting Western Central nervous system Biochemistry Nitric oxide Hypoxemia chemistry.chemical_compound Pregnancy Reference Values Internal medicine medicine biology.domesticated_animal Animals Hypoxia Molecular Biology Fetus biology Brain Lama glama respiratory tract diseases Nitric oxide synthase Fetal Diseases medicine.anatomical_structure Endocrinology chemistry Cerebral blood flow Cerebral cortex Anesthesia biology.protein Female Nitric Oxide Synthase medicine.symptom Camelids New World |
| Zdroj: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. 129:605-614 |
| ISSN: | 1095-6433 |
| DOI: | 10.1016/s1095-6433(01)00299-9 |
| Popis: | The fetal llama ( Lama glama ; a species adapted to live in chronic hypoxia in the highlands of the Andes) did not increase cerebral blood flow and reduce the brain oxygen uptake during hypoxemia. Although nitric oxide (NO) is a normal mediator in the regulation of vascular tone and synaptic transmission, NO overproduction by hypoxemia could produce neuronal damage. We hypothesized that nitric oxide synthase (NOS) activity is either maintained or reduced in the central nervous system of the llama fetuses submitted to chronic hypoxemia. Approximately 85% of the Ca 2+ -dependent NOS activity was soluble, at least 12% was associated with the mitochondrial fraction, and less than 5% remains associated with microsomes. To understand the role of NO in chronic hypoxemia, we determined the effect of 24-h hypoxemia on NOS activity in the central nervous system. No changes in activity or the subcellular distribution of NOS activity in brain tissues after hypoxemia were found. We proposed that the lack of changes in NOS activity in the llama under hypoxemia could be a cytoprotective mechanism inherent to the llama, against possible toxic effects of NO. |
| Databáze: | OpenAIRE |
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