Identification of an N-terminal TRPC2 splice variant which inhibits calcium influx
| Autor: | Jocelyn Wozney, Xin Chu, Virginia Mazack, Richard C. Stahl, Wenyi Zhang, Barbara A. Miller, Qin Tong, Dwayne L. Barber, Joseph Y. Cheung, Kathleen Conrad |
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| Rok vydání: | 2005 |
| Předmět: |
Physiology
Immunoprecipitation Molecular Sequence Data Clone (cell biology) chemistry.chemical_element Calcium Biology Green fluorescent protein Mice Animals splice Amino Acid Sequence RNA Messenger Molecular Biology TRPC Cation Channels Microscopy Confocal Membrane Proteins Cell Biology Transfection Immunohistochemistry Molecular biology Mice Inbred C57BL Blot Alternative Splicing Transmembrane domain chemistry |
| Zdroj: | Cell Calcium. 37:173-182 |
| ISSN: | 0143-4160 |
| Popis: | TRPC2 is a member of the transient receptor potential (TRP) superfamily of Ca 2+ -permeable channels expressed in nonexcitable cells. TRPC2 is involved in a number of physiological processes including sensory activation of the vomeronasal organ, sustained Ca 2+ entry in sperm, and regulation of calcium influx by erythropoietin. Here, a new splice variant of TRPC2, called “Similar to mouse TRPC2” (smTRPC2), was identified consisting of 213 amino acids, largely coincident with the N-terminus of TRPC2 clone 17. This splice variant lacks all six TRPC2 transmembrane domains and the calcium pore. Expression of smTRPC2 was found in all tissues examined by RT–PCR and in primary erythroid cells by RT–PCR and Western blotting. Confocal microscopy of CHO-S cells transfected with TRPC2 clone 14 and smTRPC2 demonstrated that TRPC2 clone 14 and smTRPC2 both localize at or near the plasma membrane and in the perinuclear region. Cell surface localization of TRPC2 was confirmed with biotinylation, and was not substantially affected by smTRPC2 expression. Coassociation of TRPC2 c14 and α with smTRPC2 was confirmed by immunoprecipitation. To examine the functional significance of smTRPC2 expression, a CHO-S model was used to study its effect on calcium influx stimulated by Epo through TRPC2. Single CHO-S cells which express transfected Epo-R were identified by detection of green fluorescent protein (GFP). Cells that express transfected TRPC2 c14 or α were identified by detection of blue fluorescent protein (BFP). [Ca] i was quantitiated with Fura Red fluorescence using digital video imaging. Epo stimulated calcium influx through TRPC2 isoforms c14 and α, which was inhibited by coexpression of smTRPC2. These data demonstrate that a short splice variant of TRPC2 exists in many cell types, which associates with and modifies the activity of functional TRPC2 splice variants. |
| Databáze: | OpenAIRE |
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