Genome-wide search for loss of heterozygosity in Burkitt lymphoma cell lines
| Autor: | Giovanni Romeo, Luo Yin, Daniel Birnbaum, Suzanne Pauly, Tetsuro Noguchi, Hagay Sobol, Gilbert M. Lenoir, Athmane Benziane, Fabienne Kerangueven, François Eisinger, Michel Longy |
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| Rok vydání: | 2002 |
| Předmět: |
Adult
Male Cancer Research Adolescent Loss of Heterozygosity Chromosomal translocation Biology Genome Loss of heterozygosity hemic and lymphatic diseases Genetics medicine Tumor Cells Cultured Humans Allele Child Gene Aged Genome Human Middle Aged medicine.disease Molecular biology Burkitt Lymphoma Lymphoma Genetic marker Child Preschool Human genome Female |
| Zdroj: | Genes, chromosomescancer. 33(2) |
| ISSN: | 1045-2257 |
| Popis: | The molecular biological characteristics of Burkitt lymphoma (BL), in addition to the presence of the Epstein-Barr virus (EBV) in some forms, relies on well-characterized alterations, such as MYC translocations and TP53 inactivations. To ascertain the number and location of other genome alterations, we used 191 polymorphic markers in a genome-wide search for loss of heterozygosity (LOH) in 31 Burkitt lymphoma cell lines and their normal counterparts. We were able to distinguish two types of altered allelic patterns: a bona fide LOH profile, indicative of deletion (LOH), and a profile indicative of increased dosage (ID). The former type was most frequent at chromosome arm 17p, most likely indicating TP53 gene inactivation. Increased dosage at 1q was found almost exclusively in non-EBV cell lines (P < 0.00004) and correlated well with karyotypic abnormalities affecting region 1q21-25. Our results suggest that a gene important for BL pathogenesis is located in region 1q21-25 and that the activation of this gene mimics the effects of EBV. |
| Databáze: | OpenAIRE |
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