A human c-erbA oncogene homologue is closely proximal to the chromosome 17 breakpoint in acute promyelocytic leukemia
| Autor: | A I Dayton, J R Selden, G Laws, D J Dorney, J Finan, P Tripputi, B S Emanuel, G Rovera, P C Nowell, C M Croce |
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| Rok vydání: | 1984 |
| Předmět: |
Acute promyelocytic leukemia
animal structures Chromosomal translocation Biology Translocation Genetic Mice medicine Animals Humans Chromosome 12 Chromosomes Human 16-18 Chromosome 7 (human) Genetics Multidisciplinary Base Sequence Nucleic Acid Hybridization Karyotype DNA Oncogenes medicine.disease Molecular biology Chromosome Banding Chromosome 17 (human) Leukemia Myeloid Acute Karyotyping Chromosome 21 Chromosome 22 Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 81(14) |
| ISSN: | 0027-8424 |
| Popis: | A human cDNA library was screened for sequences homologous to the erbA gene of avian erythroblastosis virus (AEV). One such clone, cHerbA-1, was used to map the chromosomal location of highly homologous human sequences that were found to be present on chromosome 17 as judged by Southern blot screening of a panel of mouse-human hybrid cell lines segregating human chromosomes. cHerbA-1 was hybridized in situ to metaphase chromosomes from a normal male subject and from a female patient with an acute promyelocytic leukemia (APL) having the typical t(15;17) translocation. The results localized the cellular c-erbA sequences on chromosome 17 to the q21-q24 region of normal chromosomes and indicated that the c-erbA sequences remained on the 17q- chromosome in the APL cells, suggesting that they could be assigned to the 17(q21-q22) region. For additional data, we hybridized human neoplastic cells derived from a poorly differentiated acute leukemia carrying a t(17;21) translocation with thymidine kinase (TK)-deficient LMTK- mouse cells. A resulting hybrid, containing only the 21q+ chromosome, did not have human c-erbA sequences. Since the breakpoint on 17q in this translocation was similar to that in the APL t(15;17) translocation, this supported the assignment of c-erbA to the q21-q22 region of chromosome 17. The apparent close proximity of the c-erbA sequences to the chromosomal breakpoints in these two leukemias suggests a possible role for this oncogene homologue in the development of these neoplasms. |
| Databáze: | OpenAIRE |
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