Zinc promotes clot stability by accelerating clot formation and modifying fibrin structure
| Autor: | Huayin Wu, Jeffrey I. Weitz, Sara J. Henderson, Beverly A. Leslie, David A. Weitz, Jing Xia, James C. Fredenburgh, Alan R. Stafford |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Time Factors Polymers medicine.medical_treatment chemistry.chemical_element Zinc 030204 cardiovascular system & hematology Fibrinogen Fibrin Fibrin Fibrinogen Degradation Products 03 medical and health sciences 0302 clinical medicine Thrombin Protein Domains Fibrinolysis medicine Humans Platelet activation Blood Coagulation Binding Sites biology Dose-Response Relationship Drug Factor XIII Chemistry Coagulants Batroxobin Hematology Fibrin Monomer 030104 developmental biology Biochemistry biology.protein Biophysics Microscopy Electron Scanning Rheology circulatory and respiratory physiology medicine.drug |
| Zdroj: | Thrombosis and haemostasis. 115(3) |
| ISSN: | 2567-689X |
| Popis: | SummaryZinc released from activated platelets binds fibrin(ogen) and attenuates fibrinolysis. Although zinc also affects clot formation, the mechanism and consequences are poorly understood. To address these gaps, the effect of zinc on clot formation and structure was examined in the absence or presence of factor (F) XIII. Zinc accelerated a) plasma clotting by 1.4-fold, b) fibrinogen clotting by 3.5- and 2.3-fold in the absence or presence of FXIII, respectively, c) fragment X clotting by 1.3-fold, and d) polymerisation of fibrin monomers generated with thrombin or batroxobin by 2.5– and 1.8-fold, respectively. Whereas absorbance increased up to 3.3-fold when fibrinogen was clotted in the presence of zinc, absorbance of fragment X clots was unaffected by zinc, consistent with reports that zinc binds to the αC-domain of fibrin(ogen). Scanning electron microscopic analysis revealed a twofold increase in fibre diameter in the presence of zinc and in permeability studies, zinc increased clot porosity by 30-fold with or without FXIII. Whereas FXIII increased clot stiffness from 128 ± 19 Pa to 415 ± 27 Pa in rheological analyses, zinc reduced clot stiffness by 10– and 8.5-fold in the absence and presence of FXIII, respectively. Clots formed in the presence of zinc were more stable and resisted rupture with or without FXIII. Therefore, zinc accelerates clotting and reduces fibrin clot stiffness in a FXIII-independent manner, suggesting that zinc may work in concert with FXIII to modulate clot strength and stability. |
| Databáze: | OpenAIRE |
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