Genomic Analysis of Multiresistant Staphylococcus capitis Associated with Neonatal Sepsis
| Autor: | Deborah A Williamson, James E. Ussher, Thomas V. Riley, Helen Heffernan, Sarah L. Baines, Anders Gonçalves da Silva, Glen P. Carter, Timothy P. Stinear, Roland S. Broadbent, Ian R. Monk, Benjamin P Howden, Antje van der Linden, Jean Lee |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
coagulase-negative staphylococci Coagulase plasmids health care facilities manpower and services education 030106 microbiology Drug resistance Staphylococcal infections Microbiology Epidemiology and Surveillance 03 medical and health sciences Staphylococcus capitis Antibiotic resistance multidrug resistance Intensive care Drug Resistance Multiple Bacterial Intensive Care Units Neonatal medicine Humans Pharmacology (medical) Blood culture Pharmacology Neonatal sepsis medicine.diagnostic_test biology Infant Newborn Genomics Staphylococcal Infections medicine.disease biology.organism_classification neonates 3. Good health Anti-Bacterial Agents Infectious Diseases Neonatal Sepsis New Zealand |
| Zdroj: | Antimicrobial Agents and Chemotherapy |
| ISSN: | 1098-6596 0066-4804 |
| Popis: | Coagulase-negative staphylococci (CoNS), such as Staphylococcus capitis, are major causes of bloodstream infections in neonatal intensive care units (NICUs). Recently, a distinct clone of S. capitis (designated S. capitis NRCS-A) has emerged as an important pathogen in NICUs internationally. Coagulase-negative staphylococci (CoNS), such as Staphylococcus capitis, are major causes of bloodstream infections in neonatal intensive care units (NICUs). Recently, a distinct clone of S. capitis (designated S. capitis NRCS-A) has emerged as an important pathogen in NICUs internationally. Here, 122 S. capitis isolates from New Zealand (NZ) underwent whole-genome sequencing (WGS), and these data were supplemented with publicly available S. capitis sequence reads. Phylogenetic and comparative genomic analyses were performed, as were phenotypic assessments of antimicrobial resistance, biofilm formation, and plasmid segregational stability on representative isolates. A distinct lineage of S. capitis was identified in NZ associated with neonates and the NICU environment. Isolates from this lineage produced increased levels of biofilm, displayed higher levels of tolerance to chlorhexidine, and were multidrug resistant. Although similar to globally circulating NICU-associated S. capitis strains at a core-genome level, NZ NICU S. capitis isolates carried a novel stably maintained multidrug-resistant plasmid that was not present in non-NICU isolates. Neonatal blood culture isolates were indistinguishable from environmental S. capitis isolates found on fomites, such as stethoscopes and neonatal incubators, but were generally distinct from those isolates carried by NICU staff. This work implicates the NICU environment as a potential reservoir for neonatal sepsis caused by S. capitis and highlights the capacity of genomics-based tracking and surveillance to inform future hospital infection control practices aimed at containing the spread of this important neonatal pathogen. |
| Databáze: | OpenAIRE |
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