Hexosamine interference with the determination of protein by the Lowry procedure
| Autor: | David H. Berg |
|---|---|
| Rok vydání: | 1971 |
| Předmět: |
chemistry.chemical_classification
Glucosamine Chromatography Chemistry Biophysics Proteins Protein level Galactosamine Hexosamines Serum Albumin Bovine Cell Biology Acetates Sulfuric Acids Biochemistry chemistry.chemical_compound Lowry protein assay Methods Animals Chondroitin Cattle Molecular Biology |
| Zdroj: | Analytical Biochemistry. 42:505-508 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(71)90065-0 |
| Popis: | 1. 1. d -glucosamine and d -galactosamine and their corresponding N-acetyl derivatives interfere in the determination of protein by the Lowry assay. 2. 2. At the level of 0.5 mg/ml of free hexosamine, d -glucosamine gave an apparent protein level of 0.055 mg/ml and d -galactosamine, 0.070 mg/ml. At 2.0 mg/ml hexosamine, the respective values were 0.128 mg/ml and 0.220 mg/ml. 3. 3. The N-acetyl derivatives yielded approximately one-fifth of these values. 4. 4. Chondroitin 6-sulfate does not interfere with the Lowry protein assay. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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