First Survey of Metallo-β-Lactamases in Clinical Isolates of Pseudomonas aeruginosa in a German University Hospital
| Autor: | Doris Turnwald, Matthias Frosch, Marianne Abele-Horn, Anett Oesterlein, Heike Claus, Johannes Elias, Christoph Schoen, Giuseppe Valenza, Kathrin Engelhardt, Biju Joseph |
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| Rok vydání: | 2010 |
| Předmět: |
Imipenem
medicine.drug_class medicine.medical_treatment Cephalosporin Ceftazidime chemical and pharmacologic phenomena Microbial Sensitivity Tests Biology medicine.disease_cause beta-Lactamases Microbiology Hospitals University Plasmid Mechanisms of Resistance Germany Drug Resistance Bacterial medicine Humans Pseudomonas Infections Pharmacology (medical) Pharmacology Pseudomonas aeruginosa biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Anti-Bacterial Agents Infectious Diseases Pseudomonadales Beta-lactamase medicine.drug Pseudomonadaceae |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 54:3493-3497 |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.00080-10 |
| Popis: | A total of 489 clinical isolates of Pseudomonas aeruginosa was investigated for metallo-β-lactamase (MBL) production. Molecular analysis detected a bla VIM-1 gene in the chromosome of one isolate and a bla VIM-2 gene carried on the plasmid in seven isolates. Moreover, we showed that an initial screening by combined susceptibility testing of imipenem and ceftazidime followed by a confirmatory EDTA combination disk test represents a valid alternative to the molecular investigation of MBL genes, making MBL detection possible in routine diagnostic laboratories. |
| Databáze: | OpenAIRE |
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