Synergistic effect of docosahexaenoic acid or conjugated linoleic acid with caffeic acid on ameliorating oxidative stress of HepG2 cells
| Autor: | Junrui Wu, Guoyang Pan, Cong Wang, Xue Luo, Yuanyuan Bian, Delun Mu, Yumeng Liu, Haisu Shi |
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| Rok vydání: | 2021 |
| Předmět: |
Antioxidant
Carcinoma Hepatocellular Docosahexaenoic Acids 030309 nutrition & dietetics Conjugated linoleic acid medicine.medical_treatment medicine.disease_cause 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology Caffeic Acids Caffeic acid medicine Humans Linoleic Acids Conjugated chemistry.chemical_classification 0303 health sciences Reactive oxygen species Glutathione peroxidase Liver Neoplasms food and beverages Drug Synergism 04 agricultural and veterinary sciences Hep G2 Cells Malondialdehyde 040401 food science Oxidative Stress chemistry Biochemistry Docosahexaenoic acid lipids (amino acids peptides and proteins) Drug Therapy Combination Oxidative stress Food Science |
| Zdroj: | Journal of food scienceREFERENCES. 86(7) |
| ISSN: | 1750-3841 |
| Popis: | Exploring the synergistic effect of docosahexaenoic acid (DHA) or conjugated linoleic acid (CLA) with caffeic acid (CA) on ameliorating oxidative stress, thereby introducing CA to DHA or CLA will contribute significantly to enhance the bioactivity. We observed that DHA or CLA with CA promoted the recovery of intact individual morphology and the decline of cavities inside the nucleus and apoptosis under the observation of confocal laser scanning microscopy and fluorescent inverted microscope. The activity of intracellular antioxidant enzymes catalase (CAT) and glutathione peroxidase (GSH-Px), lactate dehydrogenase (LDH) leakage, pyruvate and malondialdehyde and reactive oxygen species (ROS), cellular morphology, and cell cycle were analyzed. Our results showed that DHA or CLA with CA enhanced the activity of CAT and GSH-Px, decreased LDH leakage and the number of apoptotic, significantly inhibited (ROS-induced cellular injury. Cell arrest in G1 and G2 phase during cell mitosis was reduced by the measurement of flow cytometry. DHA or CLA combined with CA could markedly strengthen the free radical scavenging and endogenous antioxidant defense capacity on HepG2 cells. This study provides a new direction in the application of synergies to antioxidant compounds. PRACTICAL APPLICATION: Caffeic acid (CA) can synergize with docosahexaenoic acid (DHA) or conjugated linoleic acid (CLA) to enhance antioxidant capacity. This study highlighted an effect of ameliorating oxidative stress injury DHA or CLA with CA on HepG2 cells. The data indicated that DHA or CLA with CA might be used to relieve oxidative stress damage. |
| Databáze: | OpenAIRE |
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