Analysis of Major Withanolides inPhysalis longifoliaNutt. by HPLC-PDA
Autor: | Kelly Kindscher, Robert J. Gallagher, Barbara N. Timmermann, Huaping Zhang, Cong-Mei Cao |
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Rok vydání: | 2014 |
Předmět: |
Liquid Chromatography
Detection limit Acetonitriles Chromatography Physalis biology Chemistry Elution Calibration curve Extraction (chemistry) Reproducibility of Results General Medicine biology.organism_classification Antineoplastic Agents Phytogenic Analytical Chemistry Limit of Detection Calibration Physalis longifolia Quantitative assessment Hplc pda Withanolides Chromatography High Pressure Liquid |
Zdroj: | Journal of Chromatographic Science. 53:1044-1047 |
ISSN: | 1945-239X 0021-9665 |
DOI: | 10.1093/chromsci/bmu162 |
Popis: | An analytical method based on high-performance liquid chromatography-photodiode array detection was developed for the simultaneous determination of three anti-proliferative withanolides [withalongolide A ( 1: ), withaferin A ( 2: ) and withalongolide B ( 3: )] present in the aboveground biomass of the long-leaf groundcherry, Physalis longifolia. This method was achieved by biomass extraction followed by chromatographic separation on C18 column eluted with a gradient acetonitrile-water mobile phase. Calibration curves produced satisfactory linear regression (r(2) > 0.9995) for each examined sample. The method was also validated for accuracy, precision and limits of detection and quantification. Such an approach is applicable for the rapid detection and quantitative assessment of withanolides in various P. longifolia accessions. |
Databáze: | OpenAIRE |
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