Miniaturized Protein Microarray with Internal Calibration as Point-of-Care Device for Diagnosis of Neonatal Sepsis
| Autor: | Claudia Preininger, Ursula Sauer, Patricia Buchegger, Hedvig Toth-Székély |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Streptavidin
neonatal sepsis Point-of-Care Systems Point-of-care testing Protein Array Analysis Biosensing Techniques lcsh:Chemical technology streptavidin coated magnetic particles Sensitivity and Specificity Biochemistry Article Analytical Chemistry Sepsis Andrology chemistry.chemical_compound medicine Humans lcsh:TP1-1185 internal calibration Electrical and Electronic Engineering Instrumentation Immunoassay Miniaturization Neonatal sepsis medicine.diagnostic_test business.industry Infant Newborn Reproducibility of Results Neopterin Equipment Design protein microarray miniaturization point-of-care testing medicine.disease Blood proteins Atomic and Molecular Physics and Optics Equipment Failure Analysis chemistry Calibration Immunology Protein microarray Cytokines business Biomarkers |
| Zdroj: | Sensors; Volume 12; Issue 2; Pages: 1494-1508 Sensors (Basel, Switzerland) Sensors, Vol 12, Iss 2, Pp 1494-1508 (2012) |
| ISSN: | 1424-8220 |
| Popis: | Neonatal sepsis is still a leading cause of death among newborns. Therefore a protein-microarray for point-of-care testing that simultaneously quantifies the sepsis associated serum proteins IL-6, IL-8, IL-10, TNF alpha, S-100, PCT, E-Selectin, CRP and Neopterin has been developed. The chip works with only a 4 µL patient serum sample and hence minimizes excessive blood withdrawal from newborns. The 4 µL patient samples are diluted with 36 µL assay buffer and distributed to four slides for repetitive measurements. Streptavidin coated magnetic particles that act as distinct stirring detection components are added, not only to stir the sample, but also to detect antibody antigen binding events. We demonstrate that the test is complete within 2.5 h using a single step assay. S-100 conjugated to BSA is spotted in increasing concentrations to create an internal calibration. The presented low volume protein-chip fulfills the requirements of point-of-care testing for accurate and repeatable (CV < 14%) quantification of serum proteins for the diagnosis of neonatal sepsis. |
| Databáze: | OpenAIRE |
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