A simple turbidimetric method for the determination of microgram quantities of Trition X-100
| Autor: | Kazuhiko Ishihara, Susumu Ohara, Kyoko Hotta, Yoshiteru Azuumi, Kazunari Yoshida |
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| Rok vydání: | 1980 |
| Předmět: |
Residue (complex analysis)
Chromatography Ethanol Octoxynol Chemistry Microchemistry Microgram Chondroitin Sulfates Biophysics Serum Albumin Bovine DNA Cell Biology Biochemistry Polyethylene Glycols chemistry.chemical_compound Nephelometry and Turbidimetry Solubilization Phosphatidylcholines Nucleic acid Phenol Turbidity Molecular Biology |
| Zdroj: | Analytical Biochemistry. 108:162-165 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(80)90707-1 |
| Popis: | A simple and sensitive procedure for the quantitative estimation of Triton X-100 is described. The method is based on the formation of turbidity from Triton X-100 with phenol. The turbidity is proportional to Triton X-100 in a range of 20–80 μg/ml. Protein, mucopolysaccharide, and nucleic acid do not interfere in this turbidity formation. The method is especially useful for detection of the residue after the removal of Triton X-100 from solubilized samples. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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