PulmoStent: In Vitro to In Vivo Evaluation of a Tissue Engineered Endobronchial Stent
| Autor: | Thiebes, Anja Lena, Kelly, Nicola, Sweeney, Caoimhe A., McGrath, Donnacha J., Clauser, Johanna, Kurtenbach, Kathrin, Gesche, Valentine N., Chen, Weiluan, Kok, Robbert Jan, Steinseifer, Ulrich, Bruzzi, Mark, O’Brien, Barry J., McHugh, Peter E., Jockenhoevel, Stefan, Cornelissen, Christian G., Pharmaceutics, Afd Pharmaceutics |
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| Přispěvatelé: | Pharmaceutics, Afd Pharmaceutics, AMIBM, RS: FSE AMIBM, Sciences, RS: FSE Sciences, Biobased Materials, RS: FSE Biobased Materials |
| Rok vydání: | 2016 |
| Předmět: |
Animal trial
medicine.medical_specialty Airway stenting Mucociliary clearance medicine.medical_treatment 0206 medical engineering Cell Culture Techniques Biomedical Engineering 02 engineering and technology 03 medical and health sciences Dogs 0302 clinical medicine Tissue engineering Blood vessel prosthesis In vivo Alloys Journal Article medicine Animals Stent development Sheep business.industry Endothelial Cells Granulation tissue Stent equipment and supplies medicine.disease 020601 biomedical engineering Blood Vessel Prosthesis Surgery Stenosis medicine.anatomical_structure 030228 respiratory system Female Stents business Airway Biomedical engineering |
| Zdroj: | Annals of Biomedical Engineering, 45(4), 873. Springer Netherlands Annals of Biomedical Engineering, 45(4), 873-883. Springer, Cham |
| ISSN: | 1573-9686 0090-6964 |
| DOI: | 10.1007/s10439-016-1737-9 |
| Popis: | Currently, there is no optimal treatment available for end stage tumour patients with airway stenosis. The PulmoStent concept aims on overcoming current hurdles in airway stenting by combining a nitinol stent with a nutrient-permeable membrane, which prevents tumour ingrowth. Respiratory epithelial cells can be seeded onto the cover to restore mucociliary clearance. In this study, a novel hand-braided dog bone stent was developed, covered with a polycarbonate urethane nonwoven and mechanically tested. Design and manufacturing of stent and cover were improved in an iterative process according to predefined requirements for permeability and mechanical properties and finally tested in a proof of concept animal study in sheep for up to 24 weeks. In each animal two stents were implanted, one of which was cell-seeded by endoscopic spraying in situ. We demonstrated the suitability of this membrane for our concept by glucose transport testing and in vitro culture of respiratory epithelial cells. In the animal study, no migration occurred in any of the twelve stents. There was only mild granulation tissue formation and tissue reaction; no severe mucus plugging was observed. Thus, the PulmoStent concept might be a step forward for palliative treatment of airway stenosis with a biohybrid stent device. |
| Databáze: | OpenAIRE |
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