V.D.R.L. Chancroid Studies
| Autor: | Sidney Olansky, David C. Albritton, Wilbur E. Deacon, William Kaplan |
|---|---|
| Rok vydání: | 1956 |
| Předmět: |
medicine.medical_specialty
Bacilli Gonorrhea Topical antibiotics Dermatology urologic and male genital diseases Biochemistry Microbiology Antigen medicine Potency Intradermal injection Molecular Biology Whole blood biology Inoculation business.industry Cell Biology bacterial infections and mycoses medicine.disease biology.organism_classification Virology Chancroid female genital diseases and pregnancy complications Vaccination Hemophilus ducreyi Immunology Syphilis business |
| Zdroj: | Journal of Investigative Dermatology. 26:415-419 |
| ISSN: | 0022-202X |
| DOI: | 10.1038/jid.1956.53 |
| Popis: | The intracutaneous injection of killed suspensions of Hemophilus ducreyi was shown by Ito (1) to produce a focal reaction of induration and hyperemia in human patients with chancroidal infection. This finding led to the employment of such vaccines for diagnostic purposes. Although opinions have varied as to the sensitivity and specificity of this test, Ducrey antigens have been extensively employed, especially in foreign countries, as diagnostic aids, and they have been generally recognized to be of value (2, 3, 4). In this country in the past, diagnostic antigens have been successfully prepared from organisms cultivated in media containing liquid defibrinated rabbit blood (5, 6). In the preparation of the vaccine, lysis of red blood cells and removal of hemoglobin could be accomplished by washing the bacterial suspensions several times in sterile distilled water. However, the final product contained rabbit red cell debris, in addition to the bacilli. Beeson et at. (7) reported that a medium containing serum or ascitic fluid would support the growth of this organism. Later, Deacon et at. (8) confirmed the results of these workers, and found that rabbit serum could be substituted for whole blood in cultivation of H. ducreyi. Feiner et at. (9) reported that rabbits infected by the intradermal injection of living Ducrey bacilli subsequently developed cutaneous sensitivity to the inoculation of heat killed organisms. These workers employed both commercial vaccine (Lederle) and their own antigens and found that previously injected animals reacted in a manner similar to man. The homemade preparations were made from organisms grown in serum broth. Until recently a commercially prepared antigen (Lederle) was available for use in this country. This company has ceased to manufacture the vaccine, and until such time as commercial products are again available, prospective users of Ducrey antigens will have to employ homemade preparations. The purpose of this communication is to report the results of a study in which rabbits were employed to test the potency of various vaccines prepared in our laboratory. These antigens were made from both virulent and avirulent strains of H. ducreyi cultivated in a liquid medium enriched with rabbit serum. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|