Copy number gains on 22q13 in adenoid cystic carcinoma of the salivary gland revealed by comparative genomic hybridization and tissue microarray analysis
| Autor: | Sibylle Ohl, Bert Burke, Peter Lichter, Christa Flechtenmacher, Stefan Hassfeld, Axel Walch, Frauke Devens, Stefan Joos, Christof Hofele, Kolja Freier |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
Male Cancer Research Pathology medicine.medical_specialty Adenoid cystic carcinoma Chromosomes Human Pair 22 Gene Dosage In situ hybridization Biology Proto-Oncogene Mas Gene dosage Genetics Carcinoma medicine Humans Molecular Biology In Situ Hybridization Fluorescence Aged Aged 80 and over Chromosome Aberrations Tissue microarray medicine.diagnostic_test Microarray analysis techniques Nucleic Acid Hybridization DNA Neoplasm Middle Aged Microarray Analysis Salivary Gland Neoplasms medicine.disease Carcinoma Adenoid Cystic stomatognathic diseases Female Comparative genomic hybridization Fluorescence in situ hybridization |
| Zdroj: | Cancer Genetics and Cytogenetics. 159:89-95 |
| ISSN: | 0165-4608 |
| DOI: | 10.1016/j.cancergencyto.2004.09.007 |
| Popis: | Adenoid cystic carcinoma (ACC) of the salivary gland is a neoplasm characterized by slow but inevitable local progression and terminal hematogenous metastasis. To detect novel imbalanced chromosomal regions associated with tumorigenesis, we used chromosomal comparative genomic hybridization to screen 27 ACC. The most common aberration was copy number gain of 22q13 (nine cases) followed by gains of 16p (seven cases) and 17q (four cases) and copy number losses on 6q (six cases). To further delineate the prevalence of 22q13 copy number gains in ACC, fluorescence in situ hybridization was performed for five bacterial/phage artificial chromosome (BAC/PAC) probes from the 22q13 consensus region with 57 ACC on a tissue microarray. The overall prevalence of copy number gains on 22q13 was 30% of the tumors in the fluorescence in situ hybridization analysis, irrespective of histologic differentiation (cribriform/tubular vs. solid) or tumor event (primary vs. recurrent). We therefore assume that copy number gain of 22q13 is a novel frequent finding in ACC that may be involved in the initial pathogenesis of this neoplasm by proto-oncogene activation. |
| Databáze: | OpenAIRE |
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