Light Energy Conversion Surface with Gold Dendritic Nanoforests/Si Chip for Plasmonic Polymerase Chain Reaction
| Autor: | Chih-Chieh Yeh, Ming-Hua Shiao, Yung-Sheng Lin, Chia-Hsien Hsu, Yu-Cheng Chiang, Jyh Jian Chen, Hung Ji Huang, Shu-Ling Huang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Materials science
Silicon polymerase chain reaction chemistry.chemical_element 02 engineering and technology 010402 general chemistry lcsh:Chemical technology 01 natural sciences Biochemistry Article Salmonella spp. DNA Analytical Chemistry law.invention plasmon law lcsh:TP1-1185 Electrical and Electronic Engineering Absorption (electromagnetic radiation) Instrumentation Plasmon Gel electrophoresis Thermal cycler business.industry silicon gold 021001 nanoscience & nanotechnology Chip Atomic and Molecular Physics and Optics 0104 chemical sciences Halogen lamp chemistry salmonella spp. dna Optoelectronics dendritic 0210 nano-technology business Localized surface plasmon |
| Zdroj: | Sensors Volume 20 Issue 5 Sensors, Vol 20, Iss 5, p 1293 (2020) Sensors (Basel, Switzerland) |
| ISSN: | 1424-8220 |
| DOI: | 10.3390/s20051293 |
| Popis: | Surfaces with gold dendritic nanoforests (Au DNFs) on Si chips demonstrate broadband-light absorption. This study is the first to utilize localized surface plasmons of Au DNFs/Si chips for polymerase chain reaction (PCR) applications. A convenient halogen lamp was used as the heating source to illuminate the Au DNFs/Si chip for PCR. A detection target of Salmonella spp. DNA fragments was reproduced in this plasmonic PCR chip system. By semi-quantitation in gel electrophoresis analysis, the plasmonic PCR with 30 cycles and a largely reduced processing time provided results comparable with those of a commercial PCR thermal cycler with 40 cycles in more than 1 h. In the presence of an Au DNFs/Si chip, the plasmonic PCR provides superior results in a short processing time. |
| Databáze: | OpenAIRE |
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