Analysis of the brain mural cell transcriptome
| Autor: | Annika Keller, Maarja Andaloussi Mäe, Thibaud Lebouvier, Bongnam Jung, Elisabeth Raschperger, Jennifer J. Hofmann, Liqun He, Michael Vanlandewijck, Koji Ando, Christer Betsholtz |
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| Přispěvatelé: | University of Zurich, Betsholtz, Christer |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
In silico Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology) Molecular Biology Microbiology Biochemistry or Biopharmacy) 610 Medicine & health In situ hybridization Biology Mural cell Article Transcriptome 03 medical and health sciences Mice 10180 Clinic for Neurosurgery medicine Animals Cardiac and Cardiovascular Systems Gene Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi) molekylärbiologi mikrobiologi biokemi eller biofarmaci) 1000 Multidisciplinary Multidisciplinary Kardiologi Staining and Labeling Sequence Analysis RNA Gene Expression Profiling Brain Cell sorting Flow Cytometry Cell biology Gene expression profiling 030104 developmental biology medicine.anatomical_structure Microvessels Pericyte Pericytes |
| Zdroj: | Scientific Reports |
| Popis: | Pericytes, the mural cells of blood microvessels, regulate microvascular development and function and have been implicated in many brain diseases. However, due to a paucity of defining markers, pericyte identification and functional characterization remain ambiguous and data interpretation problematic. In mice carrying two transgenic reporters, Pdgfrb-eGFP and NG2-DsRed, we found that double-positive cells were vascular mural cells, while the single reporters marked additional, but non-overlapping, neuroglial cells. Double-positive cells were isolated by fluorescence-activated cell sorting (FACS) and analyzed by RNA sequencing. To reveal defining patterns of mural cell transcripts, we compared the RNA sequencing data with data from four previously published studies. The meta-analysis provided a conservative catalogue of 260 brain mural cell-enriched gene transcripts. We validated pericyte-specific expression of two novel markers, vitronectin (Vtn) and interferon-induced transmembrane protein 1 (Ifitm1), using fluorescent in situ hybridization and immunohistochemistry. We further analyzed signaling pathways and interaction networks of the pericyte-enriched genes in silico. This work provides novel insight into the molecular composition of brain mural cells. The reported gene catalogue facilitates identification of brain pericytes by providing numerous new candidate marker genes and is a rich source for new hypotheses for future studies of brain mural cell physiology and pathophysiology. |
| Databáze: | OpenAIRE |
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