Generation of Thyroid Tissues From Embryonic Stem Cells via Blastocyst Complementation In Vivo
| Autor: | Qiliang Zhou, Qingsong Ran, Yingchun Li, Yasuo Saijo, Kanako Oda, Kenji Sakimura, Manabu Abe, Toshikuni Sasaoka, Xulu Ye, Akihiro Yasue, Yoichi Ajioka |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities endocrine system endocrine system diseases Endocrinology Diabetes and Metabolism Thyroid Gland Biology lcsh:Diseases of the endocrine glands. Clinical endocrinology Mice Endocrinology In vivo Pregnancy medicine Animals Exome Blastocyst blastocyst complementation Induced pluripotent stem cell thyroid generation Original Research Mice Knockout Mice Inbred ICR FGF10 lcsh:RC648-665 Fgf10 Chimera Regeneration (biology) Thyroid Mouse Embryonic Stem Cells X-Ray Microtomography embryonic stem cells Embryonic stem cell Cell biology Complementation Mice Inbred C57BL stomatognathic diseases medicine.anatomical_structure Mutation Thyroid Dysgenesis Female pluripotent stem cells Fibroblast Growth Factor 10 |
| Zdroj: | Frontiers in Endocrinology Frontiers in Endocrinology, Vol 11 (2020) |
| ISSN: | 1664-2392 |
| Popis: | The generation of mature, functional, thyroid follicular cells from pluripotent stem cells would potentially provide a therapeutic benefit for patients with hypothyroidism, butin vitrodifferentiation remains difficult. We earlier reported thein vivogeneration of lung organsviablastocyst complementation in fibroblast growth factor 10 (Fgf10), compound, heterozygous mutant (Fgf10Ex1mut/Ex3mut) mice. Fgf10 also plays an essential role in thyroid development and branching morphogenesis, but any role thereof in thyroid organogenesis remains unclear. Here, we report that the thyroids ofFgf10Ex1mut/Ex3mutmice exhibit severe hypoplasia, and we generate thyroid tissues from mouse embryonic stem cells (ESCs) inFgf10Ex1mut/Ex3mutmiceviablastocyst complementation. The tissues were morphologically normal and physiologically functional. The thyroid follicular cells ofFgf10Ex1mut/Ex3mutchimeric mice were derived largely from GFP-positive mouse ESCs although the recipient cells were mixed. Thyroid generationin vivo viablastocyst complementation will aid functional thyroid regeneration. |
| Databáze: | OpenAIRE |
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