Characterization of carotenoid, vitamin A, and alpha-tocopheral levels in human lung tissue and pulmonary macrophages
| Autor: | A M Van Bennekum, Carrie A. Redlich, R B Ponn, J N Grauer, William S. Blaner, S L Clever |
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| Rok vydání: | 1996 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine Vitamin medicine.medical_specialty Lung Neoplasms medicine.medical_treatment Critical Care and Intensive Care Medicine chemistry.chemical_compound Internal medicine Macrophages Alveolar medicine Humans Vitamin E Respiratory system Vitamin A Lung Carotenoid Chromatography High Pressure Liquid Aged chemistry.chemical_classification medicine.diagnostic_test business.industry Retinol Metabolism Middle Aged respiratory system beta Carotene Diet respiratory tract diseases Bronchoalveolar lavage Endocrinology medicine.anatomical_structure chemistry Female business Bronchoalveolar Lavage Fluid |
| Zdroj: | American Journal of Respiratory and Critical Care Medicine. 154:1436-1443 |
| ISSN: | 1535-4970 1073-449X |
| DOI: | 10.1164/ajrccm.154.5.8912761 |
| Popis: | Increasing evidence suggests that beta-carotene, retinol (vitamin A), and alpha-tocopheral (vitamin E) may have important protective effects in the lung. However, surprisingly little is known about their storage and metabolism in human lung. Levels of beta-carotene, retinol, and alpha-tocopherol in human lung tissues and bronchoalveolar lavage (BAL) cells were determined with reverse-phase high-pressure liquid chromatography (HPLC). Fresh lung tissue, serum, and dietary questionnaires were obtained from 21 patients undergoing open lung surgery, and BAL cells from 12 of these patients. Dietary and serum levels of carotenoids, beta-carotene, retinol, and alpha-tocopherol were consistent with previously reported values. Lung tissue levels of total carotenoids, beta-carotene, retinol, and alpha-tocopherol were respectively 0.34 +/- 0.36 microg/g, 0.13 +/- 0.27 microg/g, 0.15 +/- 0.06 microg/g, and 9.60 +/- 4.86 microg/g tissue. Levels of these nutrients were also measured in BAL cells to establish potential markers for their lung tissue levels. Correlations between serum, BAL-cell, tissue, and dietary levels of the nutrients were determined. Lung tissue levels of total carotenoids, beta-carotene, and alpha-tocopherol, but not retinol, correlated well with their serum levels. Lung tissue levels of retinol and alpha-tocopherol correlated with their BAL-cell levels. These studies demonstrate quantifiable levels of retinol, alpha-tocopherol, and total carotenoids or beta-carotene in human lung tissue and BAL cells, and show that serum and/or BAL-cell levels of these nutrients can potentially be used to predict their lung tissue levels. |
| Databáze: | OpenAIRE |
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