Cow teat skin, a potential source of diverse microbial populations for cheese production

Autor: Marie-Christine Montel, Geneviève Gagne, Céline Delbès-Paus, Isabelle Verdier-Metz, F. Monsallier, Philippe Veisseire, Stéphanie Bornes
Přispěvatelé: Unité Mixte de Recherche Fromagère (UMRF), Institut National de la Recherche Agronomique (INRA), Laboratoire de Biologie, Université d'Auvergne - Clermont-Ferrand I (UdA)
Jazyk: angličtina
Rok vydání: 2012
Předmět:
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences
fromage
medicine.disease_cause
Applied Microbiology and Biotechnology
Cheese
Staphylococcus auricularis
RNA
Ribosomal
16S
activité microbienne
mamelle
Skin
bactérie
2. Zero hunger
0303 health sciences
Ecology
biology
Microbiology and Parasitology
food and beverages
Bacterial Infections
Biodiversity
Raw milk
Microbiologie et Parasitologie
Agricultural sciences
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
vache laitière
Nipples
Carrier State
Biotechnology
DNA
Bacterial
animal structures
Molecular Sequence Data
DNA
Ribosomal
Microbiology
Industrial Microbiology
03 medical and health sciences
Staphylococcus devriesei
medicine
Animals
030304 developmental biology
Staphylococcus arlettae
Bacteria
030306 microbiology
Pantoea
Sequence Analysis
DNA
biology.organism_classification
Aerococcus
Food Microbiology
Cattle
Streptococcus equinus
Staphylococcus
Sciences agricoles
Food Science
Zdroj: Applied and Environmental Microbiology
Applied and Environmental Microbiology, American Society for Microbiology, 2012, 78 (2), pp.326-333. ⟨10.1128/AEM.06229-11⟩
Applied and Environmental Microbiology 2 (78), 326-333. (2012)
ISSN: 0099-2240
1098-5336
DOI: 10.1128/AEM.06229-11⟩
Popis: The diversity of the microbial community on cow teat skin was evaluated using a culture-dependent method based on the use of different dairy-specific media, followed by the identification of isolates by 16S rRNA gene sequencing. This was combined with a direct molecular approach by cloning and 16S rRNA gene sequencing. This study highlighted the large diversity of the bacterial community that may be found on teat skin, where 79.8% of clones corresponded to various unidentified species as well as 66 identified species, mainly belonging to those commonly found in raw milk ( Enterococcus , Pediococcus , Enterobacter , Pantoea , Aerococcus , and Staphylococcus ). Several of them, such as nonstarter lactic acid bacteria (NSLAB), Staphylococcus , and Actinobacteria , may contribute to the development of the sensory characteristics of cheese during ripening. Therefore, teat skin could be an interesting source or vector of biodiversity for milk. Variations of microbial counts and diversity between the farms studied have been observed. Moreover, Staphylococcus auricularis , Staphylococcus devriesei , Staphylococcus arlettae , Streptococcus bovis , Streptococcus equinus , Clavibacter michiganensis , Coprococcus catus , or Arthrobacter gandavensis commensal bacteria of teat skin and teat canal, as well as human skin, are not common in milk, suggesting that there is a breakdown of microbial flow from animal to milk. It would then be interesting to thoroughly study this microbial flow from teat to milk.
Databáze: OpenAIRE
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