Ca2+ homeostasis and Ca2+ signalling in sulphur mustard-exposed normal human epidermal keratinocytes
| Autor: | William J. Smith, Marijke A.E. Mol |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
inorganic chemicals
Keratinocytes Cytoplasm Fura-2 chemistry.chemical_element Stimulation Biology Toxicology chemistry.chemical_compound Abnormal cellular physiology Mustard Gas Homeostasis Humans Cells Cultured integumentary system digestive oral and skin physiology General Medicine Sulfur Molecular biology Acute toxicity respiratory tract diseases chemistry Biochemistry Toxicity Carcinogens Calcium Signal transduction Histamine Signal Transduction |
| Zdroj: | Chemico-biological interactions. 100(1) |
| ISSN: | 0009-2797 |
| Popis: | To investigate the role of increased cytoplasmic free calcium levels ([Ca] i ) in sulphur mustard-induced cellular toxicity, the [Ca] i of human epidermal keratinocytes exposed to sulphur mustard concentrations between 50 and 800 μM were measured. No early increase in [Ca] i was observed during the first 20 min after application of the agent. Furthermore, at 3, 6 and 24 h after exposure to sulphur mustard, the [Ca] i of sulphur mustard-exposed cells differed only slightly from that of control cells. However, sulphur-mustard exposed cells responded with a weaker rise of [Ca] i upon stimulation with histamine and ATP than control cells. These results suggest that sulphur mustard can induce small increases in [Ca] i , which reflect abnormal cellular physiology rather than acute toxicity. In addition, it has been shown that sulphur mustard disturbs cellular Ca 2+ signalling in human epidermal keratinocytes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect