In vitro modelling of disease-induced changes in the diabetic wound fibroblast
| Autor: | Ferdinand V. Lali, Anthony D. Metcalfe, Parviz Sorooshian |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Chemokine
Nursing (miscellaneous) medicine.medical_treatment Disease 030204 cardiovascular system & hematology Diabetic wound Diabetes Complications 03 medical and health sciences 0302 clinical medicine Diabetes mellitus Diabetes Mellitus medicine Humans Fibroblast Skin 030304 developmental biology Wound Healing 0303 health sciences biology business.industry Fibroblasts medicine.disease Diabetic Foot In vitro medicine.anatomical_structure Cytokine Immunology biology.protein Cytokines Fundamentals and skills Chemokines Wound healing business |
| Zdroj: | Journal of Wound Care. 30:300-303 |
| ISSN: | 2052-2916 0969-0700 |
| DOI: | 10.12968/jowc.2021.30.4.300 |
| Popis: | Objective: Fibroblasts have been shown to play an increasingly important role within diabetic wounds. While several in vitro models of diabetic wound fibroblasts have been reported, none replicate the natural progression of the disease over time, recapitulating the acquisition of the diseased phenotype. Therefore, this study aimed to establish an in vitro model of the diabetic wound fibroblast through sustained exposure of healthy dermal fibroblasts to hyperglycaemia. Method: Primary human fibroblasts were isolated from discarded healthy skin tissue and were either exposed to normoglycaemic (control 5.5mM glucose) media or hyperglycaemic (25mM glucose) media for four weeks. Quantitative polymerase chain reaction was performed to measure the expression of inflammatory cytokines and chemokines. Results: In the hyperglycaemia model, stromal cell-derived factor (SDF)-1 expression remained consistently downregulated across all four weeks (pConclusion: This hyperglycaemia model may serve as a useful tool to characterise pathological changes in the diabetic wound fibroblast and help identify candidate therapeutic targets, such as SDF-1, that may reverse the pathology. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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