Incidence of GLMD-Like Symptoms on Grapevines Naturally Infected by Grapevine Pinot gris virus, Boron Content and Gene Expression Analysis of Boron Metabolism Genes
Autor: | Milan Špetík, Miroslav Baránek, Akila Berraf-Tebbal, Tomáš Kiss, Mária Kocanová, Jana Čechová, Alla Eddine Mahamedi, Jakub Pečenka, Dorota Tekielska, Eliška Hakalová, Aleš Vavřiník, Aleš Eichmeier, Kateřina Štůsková |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
inorganic chemicals Grapevine Pinot gris virus RT-PCR chemistry.chemical_element Coat protein Biology 01 natural sciences Vineyard 03 medical and health sciences Gene expression Boron Gene 030304 developmental biology 0303 health sciences RdRp MP/CP Agriculture Metabolism grapevine Horticulture qPCR chemistry gene expression Phloem boron Agronomy and Crop Science 010606 plant biology & botany |
Zdroj: | Agronomy, Vol 11, Iss 1020, p 1020 (2021) Agronomy Volume 11 Issue 6 |
ISSN: | 2073-4395 |
Popis: | Grapevine Pinot gris virus (GPGV) is considered to be a causal agent of Grapevine Leaf Mottling and Deformation (GLMD) disease that has been reported worldwide through the grapevine-growing regions. Seven grapevines that were collected from a vineyard in the Czech Republic were tested for the presence of GPGV in leaf and phloem tissues. Each of the seven grapevines was infected by GPGV, from which sic symptoms were mostly shown without a typical mottling. The phylogeny based on RNA-dependent RNA polymerase and movement/coat protein sequences indicated the same origin of the GPGV isolates. The GPGV titer was the highest in the grapevines with the highest GLMD-like symptoms however, some of the grapevines with milder GLMD-like symptoms had a lower GPGV titer than the asymptomatic grapevine. Soil analysis showed uneven boron content in the direct vicinity of the grapevines, while the boron content in the grapevines was more, even showing no boron deficiency. The quantitative analysis of selected gene expressions associated with boron efflux and transport only partially explained the boron content in the soil and grapevines and only in the grapevines growing in soils with the highest or lowest boron contents. The VvBor2 and VvNIP5 genes had a higher expression and VvNIP6 had a lower expression in the grapevine growing in the soil with the lowest boron content, while a low expression of VvBor1 and VvBor2 was observed in the grapevine that was grown in the soil with the highest boron content. |
Databáze: | OpenAIRE |
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