Ultrastructural localization of B-50/growth-associated protein-43 to anterogradely transported synaptophysin-positive and calcitonin gene-related peptide-negative vesicles in the regenerating rat sciatic nerve
| Autor: | Paul Verkade, W.G. Annaert, A.J. Verkleij, A. B. Oestreicher, W.H. Gispen |
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| Rok vydání: | 1996 |
| Předmět: |
Male
Plastic Embedding Calcitonin Gene-Related Peptide Synaptophysin Nerve Tissue Proteins Calcitonin gene-related peptide Axonal Transport GAP-43 Protein medicine Animals Axon Rats Wistar Membrane Glycoproteins biology General Neuroscience Vesicle Anatomy Immunohistochemistry Sciatic Nerve Axolemma Cell biology Nerve Regeneration Rats Microscopy Electron medicine.anatomical_structure nervous system Peripheral nervous system biology.protein Axoplasmic transport Sciatic nerve Synaptic Vesicles |
| Zdroj: | Neuroscience. 71(2) |
| ISSN: | 0306-4522 |
| Popis: | The growth-associated protein-43/B-50 (B-50/GAP-43) is conveyed from the neuronal soma into the axon by fast axonal transport and moved to the nerve terminal. To visualize and determine the type of vesicles by which B-50/GAP-43 is anterogradely transported in the regenerating rat sciatic nerve, we have investigated Lowicryl HM20 embedded nerve pieces dissected from the proximal side of a collection ligature. Ultrastructurally, numerous vesicular profiles of various sizes, tubules and mitochondria were seen to accumulate proximal to the collection ligature. Both, in unmyelinated and myelinated axons, B-50/GAP-43 immunoreactivity was associated with vesicular profiles which had a diameter of 50 nm. A fraction of the B-50/GAP-43 label co-localized with the small vesicle marker synaptophysin. Co-localization of B-50/GAP-43 was not detected with the large dense-core vesicle marker calcitonin gene-related peptide. These results indicate that, in rat sciatic nerve axons, B-50/GAP-43 is anterogradely transported in small 50 nm vesicles of the constitutive pathway. These transport vesicles were distinguished in two types. We suggest that one type carrying, both, B-50 GAP-43 and synaptophysin has as destination the nerve terminal, whereas the second type, which only contains B-50/GAP-43 and no synaptophysin, may be primarily targeted to the axolemma for local membrane fusion. |
| Databáze: | OpenAIRE |
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