Preparation of Silica Nanoparticles Loaded with Nootropics and Their In Vivo Permeation through Blood-Brain Barrier
| Autor: | Michal Oravec, Pavel Ulbrich, Pavel Suchy, Jan Triska, Radka Opatrilova, Petr Babula, Martin Kunes, Ingrid Brezaniova, Kamil Záruba, Josef Jampilek |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Drug
Male Article Subject Silicon dioxide media_common.quotation_subject Nanoparticle lcsh:Medicine Pharmacology Blood–brain barrier General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound In vivo medicine Animals Rats Wistar Nootropic Agents media_common Drug Carriers Chromatography General Immunology and Microbiology lcsh:R General Medicine Permeation Silicon Dioxide Rats medicine.anatomical_structure chemistry Blood-Brain Barrier Nanoparticles Nanocarriers Drug carrier Research Article |
| Zdroj: | BioMed Research International, Vol 2015 (2015) BioMed Research International |
| ISSN: | 2314-6133 |
| DOI: | 10.1155/2015/812673 |
| Popis: | The blood-brain barrier prevents the passage of many drugs that target the central nervous system. This paper presents the preparation and characterization of silica-based nanocarriers loaded with piracetam, pentoxifylline, and pyridoxine (drugs from the class of nootropics), which are designed to enhance the permeation of the drugs from the circulatory system through the blood-brain barrier. Their permeation was compared with non-nanoparticle drug substances (bulk materials) by means of anin vivomodel of rat brain perfusion. The size and morphology of the nanoparticles were characterized by transmission electron microscopy. The content of the drug substances in silica-based nanocarriers was analysed by elemental analysis and UV spectrometry. Microscopic analysis of visualized silica nanocarriers in the perfused brain tissue was performed. The concentration of the drug substances in the tissue was determined by means of UHPLC-DAD/HRMS LTQ Orbitrap XL. It was found that the drug substances in silica-based nanocarriers permeated through the blood brain barrier to the brain tissue, whereas bulk materials were not detected in the brain. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|