Adenovirus-mediated gene transfer of Bcl-xL impedes neurite regeneration in vitro
| Autor: | Toshiyuki Oshitari, Emiko Adachi-Usami, Seiji Okada, Takeshi Tokuhisa |
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| Rok vydání: | 2003 |
| Předmět: |
Indoles
Neurite viruses Genetic enhancement Green Fluorescent Proteins Genetic Vectors DNA Recombinant bcl-X Protein Biology Transfection medicine.disease_cause Retinal ganglion Retina Adenoviridae Mice Organ Culture Techniques In Situ Nick-End Labeling Neurites medicine Animals Enzyme Inhibitors Cells Cultured Fluorescent Dyes Neurons Cell Death Regeneration (biology) General Neuroscience Gene Transfer Techniques Immunohistochemistry Molecular biology Nerve Regeneration Rats Mice Inbred C57BL Luminescent Proteins NG-Nitroarginine Methyl Ester medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 Retinal ganglion cell Thy-1 Antigens sense organs |
| Zdroj: | NeuroReport. 14:1575-1578 |
| ISSN: | 0959-4965 |
| DOI: | 10.1097/00001756-200308260-00007 |
| Popis: | Mouse retinal explants were transfected with recombinant adenovirus vector carrying the green fluorescent protein (GFP) gene and the rat bcl-x(L) gene (Adeno-Bcl-xL) to determine its ability to protect retinal ganglion cells against apoptotic cell death and to promote retinal ganglion cell neurite regeneration. Adeno-Bcl-xL-incubated retinas had reduced apoptosis compared with controls. However, neurite regeneration in adeno-treated retinas was less than that of vector-free retina. These results suggest that the usefulness of adenovirus vectors for gene therapy for retinal ganglion cells may be limited. |
| Databáze: | OpenAIRE |
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