UT-B1 proteins in rat: tissue distribution and regulation by antidiuretic hormone in kidney
| Autor: | Pierre Ripoche, F Lasbennes, Pascal Bailly, Pierre Gane, Nathalie Roudier, J.-P. Cartron, Marie-Marcelle Trinh-Trang-Tan |
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| Rok vydání: | 2002 |
| Předmět: |
Male
medicine.medical_specialty Erythrocytes Glycosylation Physiology Urea transporter Vasopressins Molecular Sequence Data Down-Regulation Biology Testicle Kidney Renal Agents Rats Sprague-Dawley Mice Species Specificity Furosemide Internal medicine Gene expression medicine Renal medulla Animals Humans Deamino Arginine Vasopressin Amino Acid Sequence Diuretics Kidney Medulla Membrane Glycoproteins Membrane Transport Proteins SLC14A2 Immunohistochemistry Rats Mice Inbred C57BL medicine.anatomical_structure Endocrinology Polyclonal antibodies Astrocytes biology.protein Rabbits Carrier Proteins Antidiuretic |
| Zdroj: | American journal of physiology. Renal physiology. 283(5) |
| ISSN: | 1931-857X |
| Popis: | UT-B1 is the facilitated urea transporter of red blood cells (RBCs) and endothelial cells of descending vasa recta in the kidney. Immunoblotting with a polyclonal antibody against the C-ter sequence of rat UT-B1 revealed UT-B1 as both nonglycosylated (29 kDa) and N-glycosylated (47.5 and 33 kDa) proteins in RBC membranes, kidney medulla, brain, and bladder in rat. In testis, UT-B1 was expressed only as a nonglycosylated protein of 47.5 kDa. Immunocytochemistry confirmed that the location of UT-B1 is restricted to descending vasa recta. In brain, UT-B1 protein was found in astrocytes and ependymal cells. Cell bodies and perivascular end feet of astrocytes were labeled in brain cortex, whereas astrocyte cell processes were labeled in corpus callosum. Flow cytometry analysis of RBCs revealed a good cross-reactivity of the antibody with mouse and human UT-B1. UT-B1 protein expression in rat kidney medulla was downregulated greatly by long-term [deamino-Cys1,d-Arg8]vasopressin infusion and moderately by furosemide treatment. This study discloses an uneven distribution of UT-B1 protein within astrocytes and the regulation of renal UT-B1 protein by antidiuretic hormone. |
| Databáze: | OpenAIRE |
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