Mixed-subunit capsids can be assembled in vitro with coat protein subunits from two cucumoviruses

Autor:	John W. Randles, R.I.B. Francki, Bao-Dong Chen
Rok vydání:	1995
Předmět:	Antiserum Virus Assembly Protein subunit Biology Precipitin biology.organism_classification Cucumovirus Virology Molecular biology Cucumber mosaic virus Immunodiffusion Capsid Solanum lycopersicum Polyclonal antibodies Tomato aspermy virus biology.protein RNA Viral Cucumis sativus
Zdroj:	Journal of General Virology. 76:971-973
ISSN:	1465-2099 0022-1317
DOI:	10.1099/0022-1317-76-4-971
Popis:	Virus particles were reassembled in vitro from tomato aspermy virus strain V (V-TAV) RNA and a mixture of subunits prepared from V-TAV and 35S-labelled cucumber mosaic virus strain T (T-CMV). Immunodiffusion tests showed that the reassembled particles reacted with polyclonal antisera raised against both V-TAV and T-CMV. Radioactivity was found in the precipitin line formed between the reassembled particles and antiserum raised against T-CMV as well as in the precipitin line formed between the reassembled particles and antiserum raised against V-TAV. This shows that 35S-labelled T-CMV protein subunits were incorporated with V-TAV protein subunits into the same particles. Thus, coat proteins of V-TAV and T-CMV can co-assemble and form mixed-subunit capsids in vitro.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ae6ee28a78bfd5b1c2301301eec8621 https://doi.org/10.1099/0022-1317-76-4-971 Zobrazit plný text záznamu