The anther-specific protein encoded by the Brassica napus and Arabidopsis thaliana A6 gene displays similarity to beta-1,3-glucanases
| Autor: | Diane L. Hird, Sarah Smartt, Wyatt Paul, Rachel Hodge, Rod J. Scott, Dawn Worrall |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Enzyme complex
RNase P Immunoblotting Molecular Sequence Data Arabidopsis Brassica Stamen Gene Expression Plant Science Genes Plant Beta-1 adrenergic receptor Complementary DNA Tobacco Genetics Arabidopsis thaliana Fluorometry Amino Acid Sequence Cloning Molecular Gene Plant Proteins Base Sequence Sequence Homology Amino Acid biology Arabidopsis Proteins Glucan Endo-1 3-beta-D-Glucosidase beta-Glucosidase food and beverages DNA Glucan 1 3-beta-Glucosidase Cell Biology biology.organism_classification Cell biology Plants Toxic |
| Zdroj: | The Plant Journal. 4:1023-1033 |
| ISSN: | 1365-313X 0960-7412 |
| Popis: | An anther-specific Brassica napus cDNA, A6, and two corresponding Arabidopsis thaliana genes have been isolated. Sequence analyses of A6 revealed similarity to beta-1,3-glucanases. The deduced A6 protein differs from other beta-1,3-glucanases in the possession of a long C-terminus. Immunoblotting using an antibody raised to the A6 protein detects a temporal 60 kDa protein in B. napus buds, suggesting that the long C-terminal region is present in the mature protein. A6 promoter-GUS and RNase fusions demonstrate that the A6 gene is tapetum-specific and temporally expressed with a peak in activity when the plant normally expresses callase (a complex of endo- and exo-beta-1,3-glucanase activities). The sequence similarity of A6 to other beta-1,3-glucanases, coupled with the temporal and spatial expression data, suggests that A6 may be part of the callase enzyme complex. |
| Databáze: | OpenAIRE |
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