Docosahexaenoic Acid Inhibits Helicobacter pylori Growth In Vitro and Mice Gastric Mucosa Colonization
| Autor: | José Carlos Machado, Rui M. Ferreira, Marta Correia, Valérie Michel, Maria José Oliveira, Patricia Almeida Carvalho, Eliette Touati, Raquel Seruca, Michel Huerre, Ceu Figueiredo, Marie-Agnès Dillies, A. Alves de Matos |
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| Přispěvatelé: | Pathogenèse de Helicobacter, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Hospital Curry Cabral [Lisbon, Portugal], Universidade do Porto, Transcriptome et Epigénome (PF2), Institut Pasteur [Paris], Histotechnologie et Pathologie, The Fundação para a Ciência e a Tecnologia (FCT), Portugal, provided funds for this study and to Dr. Correia by a PhD fellowship BD/36689/2007. The Acções integradas Luso-Francesas (CRUP-PAULIF) Portugal and France also provided financial support (AF-8/09)., Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Universidade do Porto = University of Porto, Institut Pasteur [Paris] (IP) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Male
MESH: Helicobacter Infections/pathology MESH: Helicobacter pylori/drug effects lcsh:Medicine MESH: Anti-Bacterial Agents/pharmacology MESH: Gastric Mucosa/microbiology Biochemistry Mice [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases Gastrointestinal Infections MESH: Animals Bacterial Physiology lcsh:Science 0303 health sciences Multidisciplinary biology MESH: Helicobacter Infections/drug therapy Fatty Acids food and beverages Lipids 3. Good health Anti-Bacterial Agents Bacterial Biochemistry Bacterial Pathogens medicine.anatomical_structure Infectious Diseases MESH: Helicobacter pylori/ultrastructure MESH: Anti-Bacterial Agents/administration & dosage Docosahexaenoic acid MESH: Gastritis/pathology Gastritis Medicine lipids (amino acids peptides and proteins) medicine.symptom Research Article MESH: Gastritis/drug therapy Drugs and Devices Docosahexaenoic Acids Infectious Disease Control Inflammation Gastroenterology and Hepatology Microbiology Helicobacter Infections 03 medical and health sciences In vivo MESH: Mice Inbred C57BL Gastric mucosa medicine MESH: Gastritis/microbiology Animals Biology MESH: Mice MESH: Helicobacter Infections/microbiology 030304 developmental biology Helicobacter pylori 030306 microbiology lcsh:R Bacteriology biology.organism_classification bacterial infections and mycoses [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology In vitro MESH: Male MESH: Gastric Mucosa/pathology Mice Inbred C57BL MESH: Docosahexaenoic Acids/administration & dosage Gastric Mucosa lcsh:Q Bacteria |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2012, 7 (4), pp.e35072. ⟨10.1371/journal.pone.0035072⟩ PLoS ONE, 2012, 7 (4), pp.e35072. ⟨10.1371/journal.pone.0035072⟩ PLoS ONE, Vol 7, Iss 4, p e35072 (2012) Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| ISSN: | 1932-6203 |
| Popis: | International audience; H. pylori drug-resistant strains and non-compliance to therapy are the major causes of H. pylori eradication failure. For some bacterial species it has been demonstrated that fatty acids have a growth inhibitory effect. Our main aim was to assess the ability of docosahexaenoic acid (DHA) to inhibit H. pylori growth both in vitro and in a mouse model. The effectiveness of standard therapy (ST) in combination with DHA on H. pylori eradication and recurrence prevention success was also investigated. The effects of DHA on H. pylori growth were analyzed in an in vitro dose-response study and n in vivo model. We analized the ability of H. pylori to colonize mice gastric mucosa following DHA, ST or a combination of both treatments. Our data demonstrate that DHA decreases H. pylori growth in vitro in a dose-dependent manner. Furthermore, DHA inhibits H. pylori gastric colonization in vivo as well as decreases mouse gastric mucosa inflammation. Addition of DHA to ST was also associated with lower H. pylori infection recurrence in the mouse model. In conclusion, DHA is an inhibitor of H. pylori growth and its ability to colonize mouse stomach. DHA treatment is also associated with a lower recurrence of H. pylori infection in combination with ST. These observations pave the way to consider DHA as an adjunct agent in H. pylori eradication treatment. |
| Databáze: | OpenAIRE |
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