Distribution and partial characterization of the plasma lipoproteins in the hedgehog (Erinaceus europaeus L.), a hibernator with potential use in the study of the hormonal regulation of lipoprotein metabolism
| Autor: | Michel Saboureau, Laurence Beaubatie, P. Michel Laplaud |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Male
Very low-density lipoprotein medicine.medical_specialty Apolipoprotein B Density gradient Lipoproteins Size-exclusion chromatography Polyacrylamide Biophysics Lipoproteins VLDL Biochemistry chemistry.chemical_compound Endocrinology Species Specificity Hibernation Internal medicine medicine Animals Humans Polyacrylamide gel electrophoresis Phospholipids Triglycerides Chromatography biology Chemistry Lipoproteins LDL Molecular Weight Cholesterol Hedgehogs biology.protein Urea lipids (amino acids peptides and proteins) Lipoproteins HDL Lipoprotein |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. 752:396-406 |
| ISSN: | 0005-2760 |
| DOI: | 10.1016/0005-2760(83)90269-2 |
| Popis: | The hedgehog is a hibernator in which yearly cycles of several endocrine activities and seasonal variations of plasma lipids have already been demonstrated. We have consequently undertaken a study of plasma lipids and lipoproteins in this animal, bled during late April and May. Plasma cholesterol levels (178 +/- 30 mg/100 ml) were comparable with those in normal humans, while triacylglycerol was lower (46 +/- 17 mg/100 ml) and phospholipids higher (252 +/- 35 mg/100 ml). The main characteristics of the plasma lipoprotein spectrum, as determined by sequential and density gradient preparative ultracentrifugation, analytical ultracentrifugation and gel filtration chromatography, were (1) a low concentration of very-low-density components (d less than 1.006 g/ml, about 20 mg/100 ml); (2) a continuity between the low (1.006-1.063 g/ml) and high (1.063-1.21 g/ml) density components, the former (about 150 mg/100 ml) exhibiting a considerable heterogeneity upon polyacrylamide gel electrophoresis while the latter were largely predominating (570 mg/100 ml); (3) the presence, at a density of 1.087 g/ml, of a band migrating electrophoretically like human low-density lipoproteins, a finding consistent with the results of apolipoprotein electrophoresis, which showed the presence of a high-molecular-weight counterpart to apolipoprotein B, in both the low- and high-density ranges defined above; (4) the presence, throughout the entire density spectrum, of an apolipoprotein with molecular weight and mobility in polyacrylamide/urea gels similar to human apolipoprotein A-I, and (5) the presence of very-high-density lipoproteins (d 1.178-1.259 g/ml) responsible for the transport of approximately 15% of plasma cholesterol and 20% of phospholipids. |
| Databáze: | OpenAIRE |
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