Follicle-Stimulating Hormone Does Not Impact Male Bone Mass In Vivo or Human Male Osteoclasts In Vitro
| Autor: | Mira Susa, Frederic Morvan, Pierre Saint Mézard, Yves Seltenmeyer, Veronique Ritter, Uwe Junker, Barbara Thuering, Brigitte Fournier, Ngoc-Hong Luong-Nguyen |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Male endocrine system medicine.medical_specialty medicine.drug_class Endocrinology Diabetes and Metabolism Blotting Western Osteoclasts Biology Bone resorption Cell Line Bone remodeling Mice Follicle-stimulating hormone Endocrinology Osteoclast Internal medicine Cyclic AMP medicine Animals Humans Infusions Parenteral Orthopedics and Sports Medicine Femur RNA Messenger Cyclic AMP Response Element-Binding Protein Testosterone Oligonucleotide Array Sequence Analysis Dose-Response Relationship Drug Macrophages Infusion Pumps Implantable Mice Inbred C57BL medicine.anatomical_structure Gene Expression Regulation Estrogen Receptors FSH Cortical bone Follicle Stimulating Hormone Tomography X-Ray Computed Follicle-stimulating hormone receptor |
| Zdroj: | Calcified Tissue International. 82:383-391 |
| ISSN: | 1432-0827 0171-967X |
| Popis: | Bone loss in the elderly is mainly caused by osteoclast-induced bone resorption thought to be causally linked to the decline in estrogen and testosterone levels in females and males. Recently, involvement of follicle stimulating-hormone (FSH) in this process has been suggested to explain in part the etiology of the disease in females, whereas its role in males has never been examined. In this study, the direct impact of FSH on bone mass of 16-week-old C57BL/6J male mice by either daily intermittent application of 6 or 60 mug/kg of FSH or continuous delivery via miniosmotic pump of a dose of 6 mug/kg over the course of a month was assessed. Femoral peripheral quantitative computed tomographic and microcomputed tomographic analyses at 0, 2, and 4 weeks of FSH-treated mice did not reveal any differences in cancellous and cortical bone compared to sham-treated mice. FSH functionality was verified by demonstrating cAMP induction and activation of a cAMP-response element-containing reporter cell line by FSH. Furthermore, osteoclastogenesis from human mononuclear cell precursors and from RAW 264.7 cells was not affected by FSH (3, 10, 30 ng/mL) compared to control. No direct effect of FSH on gene regulation was observed by Affymetrix Gene Array on RAW 264.7 cells. Lastly, no expression of FSH receptor (FSHR) mRNA or FSHR was observed by quantitative polymerase chain reaction and Western blot in either human male osteoclasts or RAW 264.7 cells. These data show that FSH does not appear to modulate male bone mass regulation in vivo and does not act directly on osteoclastogenesis in vitro. |
| Databáze: | OpenAIRE |
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