Drought delays development of the sorghum root microbiome and enriches for monoderm bacteria
| Autor: | Erika M. Zink, Ling Xu, Young-Mo Kim, Cheng Gao, Yi Wang, Devon Birdseye, Julie A. Sievert, Stephanie DeGraaf, Devin Coleman-Derr, Grady Pierroz, Kristin Engbrecht, Henrik Vibe Scheller, Joy Hollingsworth, Kim K. Hixson, Christer Jansson, Jeffery A. Dahlberg, Zhaobin Dong, Peggy G. Lemaux, Tuesday Simmons, Robert B. Hutmacher, Dan Naylor, Mary Madera, John W. Taylor |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
16S microbiome drought Corrections Plant Roots Crop 03 medical and health sciences Metabolomics Bacterial Proteins Cell Wall RNA Ribosomal 16S metatranscriptome Colonization Microbiome Sorghum Ribosomal Multidisciplinary biology Bacteria Dehydration business.industry Microbiota fungi Root microbiome Bacterial food and beverages biology.organism_classification root RNA Bacterial 030104 developmental biology Agronomy Agriculture RNA ATP-Binding Cassette Transporters sorghum business |
| Zdroj: | Xu, L; Naylor, D; Dong, Z; Simmons, T; Pierroz, G; Hixson, KK; et al.(2018). Drought delays development of the sorghum root microbiome and enriches for monoderm bacteria. Proceedings of the National Academy of Sciences of the United States of America, 115(18), E4284-E4293. doi: 10.1073/pnas.1717308115. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/8n26v7bf Proceedings of the National Academy of Sciences of the United States of America, vol 115, iss 18 ResearcherID |
| Popis: | © 2018 National Academy of Sciences. All Rights Reserved. Drought stress is a major obstacle to crop productivity, and the severity and frequency of drought are expected to increase in the coming century. Certain root-associated bacteria have been shown to mitigate the negative effects of drought stress on plant growth, and manipulation of the crop microbiome is an emerging strategy for overcoming drought stress in agricultural systems, yet the effect of drought on the development of the root microbiome is poorly understood. Through 16S rRNA amplicon and metatranscriptome sequencing, as well as root metabolomics, we demonstrate that drought delays the development of the early sorghum root microbiome and causes increased abundance and activity of monoderm bacteria, which lack an outer cell membrane and contain thick cell walls. Our data suggest that altered plant metabolism and increased activity of bacterial ATP-binding cassette (ABC) transporter genes are correlated with these shifts in community composition. Finally, inoculation experiments with monoderm isolates indicate that increased colonization of the root during drought can positively impact plant growth. Collectively, these results demonstrate the role that drought plays in restructuring the root microbiome and highlight the importance of temporal sampling when studying plant-associated microbiomes. |
| Databáze: | OpenAIRE |
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