Development of a restriction length polymorphism combined with direct PCR technique to differentiate goose and Muscovy duck parvoviruses
| Autor: | Kai-Hui Hu, Shi Shaohua, Cheng Longfei, Fu Guanghua, Fu Qiuling, Chunhe Wan, Huang Yu, Chen Hongmei, Chen Cuiteng |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
040301 veterinary sciences Pcr cloning Polymerase Chain Reaction law.invention Parvoviridae Infections Parvovirus 0403 veterinary science 03 medical and health sciences chemistry.chemical_compound Goose law Polymorphism (computer science) Virology biology.animal Geese Animals Poultry Diseases restriction fragment length polymorphism Polymerase chain reaction General Veterinary biology Bird Diseases 04 agricultural and veterinary sciences direct PCR Note biology.organism_classification muscovy duck parvovirus Molecular biology genomic DNA Ducks 030104 developmental biology chemistry goose parvovirus Restriction fragment length polymorphism Polymorphism Restriction Fragment Length DNA |
| Zdroj: | The Journal of Veterinary Medical Science |
| ISSN: | 1347-7439 0916-7250 |
| DOI: | 10.1292/jvms.15-0326 |
| Popis: | A restriction fragment length polymorphism combined with direct PCR technique to differentiate goose and Muscovy duck parvoviruses (GPV and MDPV) was developed based on comparison of the NS gene of GPV and MDPV. Both GPV and MDPV genomic DNA can be amplified with 641 bp using the specific PCR primers. The PCR fragments can be cut into 463 bp and 178 bp only in the case of MDPV-derived PCR products, whereas the GPV-derived PCR products cannot. The method established in this study can be used to differentiate GPV and MDPV with high specificity and precision, by using a direct PCR kit and QuickCut enzyme, as quickly as conventional PCR. |
| Databáze: | OpenAIRE |
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