Detection of Cancer Stem Cells from the C6 Glioma Cell Line
| Autor: | Lei Zhang, Wang Dh, Qibing Huang, Xiaoyu Li, Zhong Yh, Xiaoling Lu, Xuanchen Zhou, Peng Zhao, Xinyu Wang, F J Qu |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Pathology
medicine.medical_specialty Cellular pathology Fluorescent Antibody Technique Mice Nude Nerve Tissue Proteins Biology Immunofluorescence Biochemistry Flow cytometry Nestin Mice Intermediate Filament Proteins Antigens CD Cancer stem cell Spheroids Cellular Glioma Biomarkers Tumor Tumor Cells Cultured medicine Animals AC133 Antigen Cell Proliferation Glycoproteins medicine.diagnostic_test Cell growth Biochemistry (medical) Cell Biology General Medicine Flow Cytometry medicine.disease Xenograft Model Antitumor Assays Neural stem cell Rats Neoplastic Stem Cells Cancer research Peptides |
| Zdroj: | Journal of International Medical Research. 37:503-510 |
| ISSN: | 1473-2300 0300-0605 |
| DOI: | 10.1177/147323000903700226 |
| Popis: | Various malignant cancers have been found to contain a sub-population of stem cell-like tumour cells, or cancer stem cells (CSCs), however, culture methods for CSCs and the size of the fraction of CSCs in C6, which is a commonly used glioma cell line, remain controversial. In this study, we demonstrated that the C6 cell line contains a fraction of tumour cells that can form tumour spheres in a simplified serum-free neural stem cell medium and express CD133 and nestin, which are widely-used markers for brain CSCs. Immunohistochemistry and immunofluorescence confirmed the existence of CSCs both in the C6 cell line and C6 xenografts. Flow cytometry demonstrated that 4.02% of cells in the C6 cell line and 4.21% in the C6 xenografts presented as CSCs. These results confirm the fraction of CSCs in the C6 cell line and provide a simple and effective method for isolation of CSCs to study the initiation and progression of human glioma and, possibly, other malignant tumours. |
| Databáze: | OpenAIRE |
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