Nuclear protein contents in peripheral blood mononuclear cells of trisomy 21 infants
| Autor: | Sefer Kumandaş, Turkan Patiroglu, Halil Demirtas, Zuhal Hamurcu |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Aging Pathology medicine.medical_specialty Histology Biology Peripheral blood mononuclear cell Pathology and Forensic Medicine Andrology chemistry.chemical_compound Predictive Value of Tests medicine Humans Propidium iodide Nuclear protein Child Fluorescein isothiocyanate Cell Nucleus Age Factors Infant Newborn Infant Nuclear Proteins Antigens Nuclear Cell Biology Flow Cytometry medicine.disease Up-Regulation Cell nucleus medicine.anatomical_structure chemistry Child Preschool Leukocytes Mononuclear Female Down Syndrome Chromosome 21 Trisomy Cytometry Biomarkers |
| Zdroj: | Cytometry Part B: Clinical Cytometry. :128-132 |
| ISSN: | 1552-4957 1552-4949 |
| DOI: | 10.1002/cyto.b.20387 |
| Popis: | Objective The trisomy 21 (Ts21) or Down's syndrome (DS) phenotype is assumed to occur primarily by the expression/overexpression of some genes encoded by the extra chromosome 21. It has recently been shown by AgNOR staining that babies with Ts21 have more AgNOR area (more NOR proteins) and more RNA content in their peripheral blood mononuclear cells (PBMCs) than those of controls. The aim of this study was to test whether or not the nuclear proteins content of PBMCs from trisomy 21 babies/infants is higher than that of the controls. Method For this purpose, flow cytometric measurement of the stained PBMC nuclei was used. Nuclei from PBMCs was isolated and stained with propidium iodide and fluorescein isothiocyanate (PI/FITC) for DNA and protein estimation, respectively. Results Mean nuclear protein content of Ts21's (N = 30, mean age = 3.46 ± 3.05 years old) PBMCs was found statistically higher than that of the controls (N = 33, mean age = 3.79 ± 1.93 years old) (P = 0.005, nonparametric Mann–Whitney U test for two independent variables). This means that the average nuclear protein content of PBMC from Ts21 infants is higher than that of the controls. Furthermore, there is a moderate negative correlation between the ages of the studied DS patients and the protein content in the nuclei of their PBMCs (Linear regression analysis: P = 0.002, r = −0.55). This correlation is not found with controls (P = 0.186, r = −0.24). Conclusion We have concluded that average protein content of PBMCs' nuclei from DS infants is higher than that of the controls, decreasing significantly with age. © 2007 Clinical Cytometry Society |
| Databáze: | OpenAIRE |
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