Nociceptive Signals Induce Trafficking of TRPA1 to the Plasma Membrane

Autor:	Ardem Patapoutian, Manuela Schmidt, Taryn J. Earley, Adrienne E. Dubin, Matt Petrus
Rok vydání:	2009
Předmět:	Phospholipase C Neuroscience(all) General Neuroscience food and beverages Biology MOLNEURO Exocytosis Cell biology Transient receptor potential channel SIGNALING Nociceptor Noxious stimulus CELLBIO Signal transduction Protein kinase A psychological phenomena and processes Ion channel
Zdroj:	Neuron. 64:498-509
ISSN:	0896-6273
DOI:	10.1016/j.neuron.2009.09.030
Popis:	SummaryTransient receptor potential A1 (TRPA1) ion channel senses a variety of noxious stimuli and is involved in nociception. Many TRPA1 agonists covalently modify the channel, which can lead to desensitization. The fate of modified TRPA1 and the mechanism of preserving its response to subsequent stimuli are not understood. Moreover, inflammatory signals sensitize TRPA1 by involving protein kinase A (PKA) and phospholipase C (PLC) through unknown means. We show that TRPA1-mediated nocifensive behavior can be sensitized in vivo via PKA/PLC signaling and by activating TRPA1 with the ligand mustard oil (MO). Interestingly, both stimuli increased TRPA1 membrane levels in vitro. Tetanus toxin attenuated the response to the second of two pulses of MO in neurons, suggesting that vesicle fusion increases functional surface TRPA1. Capacitance recordings suggest that MO can induce exocytosis. We propose that TRPA1 translocation to the membrane might represent one of the mechanisms controlling TRPA1 functionality upon acute activation or inflammatory signals.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a589f0e63f948b785f38ead9009d9b9 https://doi.org/10.1016/j.neuron.2009.09.030 Zobrazit plný text záznamu