Ontogenic development of 'natural' and induced plaque-forming cell isotypes in normal mice
| Autor: | Sven Pettersson, Mariana Björklund, Antonio Coutinho |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
medicine.medical_specialty
Lipopolysaccharide Immunology Immunoglobulins Bone Marrow Cells Spleen Biology Andrology Mice chemistry.chemical_compound Bone Marrow In vivo Internal medicine medicine Animals Immunology and Allergy Weaning Antibody-Producing Cells Cells Cultured B-Lymphocytes Mice Inbred C3H Age Factors T-Lymphocytes Helper-Inducer Antigens T-Independent Isotype In vitro medicine.anatomical_structure Endocrinology chemistry Polyclonal antibodies biology.protein Bone marrow |
| Zdroj: | European Journal of Immunology. 15:1003-1007 |
| ISSN: | 1521-4141 0014-2980 |
| DOI: | 10.1002/eji.1830151008 |
| Popis: | The numbers of cells and background plaque-forming cells (PFC) in the spleen of C3H/HeJ mice increase exponentially during the first 2 weeks after birth, but much slower in bone marrow (BM). IgGl and IgG2a PFC are the first non-IgM PFC detectable, while IgG3 and IgA PFC appear only around weaning. Adult-type PFC numbers and isotype pattern are present in spleen and BM at 4 and 15 weeks, respectively. Neonatal splenic C3H/Tif B cells produce non-IgM Ig classes in vitro in response to polyclonal activation by lipopolysaccharide or by helper T cells. These responses are of low magnitude during the first 2 weeks of life, but both secreted and membrane-bound IgG1 and IgG3 isotypes are detectable already a few days after birth, in a pattern that is identical to that typical of T cell-dependent or independent responses of adult cells. These results indicate full maturity of B cells in “switch” abilities already from birth, in spite of a general deficiency in terminal maturation. In addition, they demonstrate the complexity of isotype regulation in “background” antibody production in vivo. |
| Databáze: | OpenAIRE |
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