Nitrogen-Fixing Nodules Are an Important Source of Reduced Sulfur, Which Triggers Global Changes in Sulfur Metabolism in Lotus japonicus
| Autor: | Heinz Rennenberg, Cornelia Herschbach, Panagiotis Krompas, Georgios Karalias, Emmanouil Flemetakis, Chrysanthi Kalloniati, Michael K. Udvardi |
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| Rok vydání: | 2015 |
| Předmět: |
Root nodule
Lotus japonicus Sulfur metabolism Plant Science Sulfur Radioisotopes Rhizobia Bacterial Proteins Symbiosis Gene Expression Regulation Plant Nitrogen Fixation Botany Oxidoreductases Acting on Sulfur Group Donors Tissue Distribution Large-Scale Biology Article Sulfhydryl Compounds Plant Proteins biology fungi Mesorhizobium food and beverages Cell Biology Metabolism biology.organism_classification Biochemistry Lotus Nitrogen fixation Oxidoreductases Root Nodules Plant Sulfur Transcription Factors |
| Zdroj: | The Plant Cell. 27:2384-2400 |
| ISSN: | 1532-298X 1040-4651 |
| DOI: | 10.1105/tpc.15.00108 |
| Popis: | We combined transcriptomic and biochemical approaches to study rhizobial and plant sulfur (S) metabolism in nitrogen (N) fixing nodules (Fix(+)) of Lotus japonicus, as well as the link of S-metabolism to symbiotic nitrogen fixation and the effect of nodules on whole-plant S-partitioning and metabolism. Our data reveal that N-fixing nodules are thiol-rich organs. Their high adenosine 5'-phosphosulfate reductase activity and strong (35)S-flux into cysteine and its metabolites, in combination with the transcriptional upregulation of several rhizobial and plant genes involved in S-assimilation, highlight the function of nodules as an important site of S-assimilation. The higher thiol content observed in nonsymbiotic organs of N-fixing plants in comparison to uninoculated plants could not be attributed to local biosynthesis, indicating that nodules are an important source of reduced S for the plant, which triggers whole-plant reprogramming of S-metabolism. Enhanced thiol biosynthesis in nodules and their impact on the whole-plant S-economy are dampened in plants nodulated by Fix(-) mutant rhizobia, which in most respects metabolically resemble uninoculated plants, indicating a strong interdependency between N-fixation and S-assimilation. |
| Databáze: | OpenAIRE |
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