Thin cell layer cultures of Chlamydomonas reinhardtii L159I-N230Y, pgrl1 and pgr5 mutants perform enhanced hydrogen production at sunlight intensity
| Autor: | Anna Podmaniczki, Éva Herman, Szilvia Z. Tóth, Alberto Scoma, Soujanya Kuntam, Dávid Tóth, László Kovács, André Vidal-Meireles, Valéria Nagy |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Environmental Engineering Photosystem II Mutant Chlamydomonas reinhardtii Bioengineering 010501 environmental sciences Photosynthesis Photosystem I 01 natural sciences chemistry.chemical_compound Hydrogenase Hyda 010608 biotechnology Waste Management and Disposal 0105 earth and related environmental sciences biology Photosystem I Protein Complex Renewable Energy Sustainability and the Environment General Medicine biology.organism_classification Oxygen Light intensity chemistry Chlorophyll Biophysics Sunlight Biohydrogen Thin cell layer cultures Hydrogen |
| Zdroj: | Nagy, V, Podmaniczki, A, Vidal-Meireles, A, Kuntam, S, Herman, É, Kovács, L, Tóth, D, Scoma, A & Tóth, S Z 2021, ' Thin cell layer cultures of Chlamydomonas reinhardtii L159I-N230Y, pgrl1 and pgr5 mutants perform enhanced hydrogen production at sunlight intensity ', Bioresource Technology, vol. 333, 125217 . https://doi.org/10.1016/j.biortech.2021.125217 |
| DOI: | 10.1016/j.biortech.2021.125217 |
| Popis: | Photobiological hydrogen (H2) production is a promising renewable energy source. HydA hydrogenases of green algae are efficient but O2-sensitive and compete for electrons with CO2-fixation. Recently, we established a photoautotrophic H2 production system based on anaerobic induction, where the Calvin-Benson cycle is inactive and O2 scavenged by an absorbent. Here, we employed thin layer cultures, resulting in a three-fold increase in H2 production relative to bulk CC-124 cultures (50 µg chlorophyll/ml, 350 µmol photons m−2 s−1). Productivity was maintained when increasing the light intensity to 1000 µmol photons m-2s−1 and the cell density to 150 µg chlorophyll/ml. Remarkably, the L159I-N230Y photosystem II mutant and the pgrl1 photosystem I cyclic electron transport mutant produced 50% more H2 than CC-124, while the pgr5 mutant generated 250% more (1.2 ml H2/ml culture in six days). The photosynthetic apparatus of the pgr5 mutant and its in vitro HydA activity remained remarkably stable. |
| Databáze: | OpenAIRE |
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