Chronic Hypoxia Inhibits Respiratory Complex IV Activity and Disrupts Mitochondrial Dynamics in the Fetal Guinea Pig Forebrain
| Autor: | Hong Song, Jamiu Giwa-Otusajo, Tabitha M. Quebedeaux, Loren P. Thompson |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Fetus Electron Transport Complex I Guinea Pigs MFN2 Obstetrics and Gynecology Mitochondrion Intrauterine hypoxia Hypoxia (medical) Biology medicine.disease Fetal Hypoxia Mitochondrial Dynamics Guinea pig Electron Transport Complex IV Endocrinology Prosencephalon Mitochondrial biogenesis Pregnancy Internal medicine medicine Animals Female Respiratory system medicine.symptom |
| Zdroj: | Reproductive sciences (Thousand Oaks, Calif.). 29(1) |
| ISSN: | 1933-7205 |
| Popis: | Mitochondrial dysfunction is an underlying cause of childhood neurological disease secondary to the crucial role of mitochondria in proper neurodevelopment. We hypothesized that chronic intrauterine hypoxia (HPX) induces mitochondrial deficits by altering mitochondrial biogenesis and dynamics in the fetal brain. Pregnant guinea pigs were exposed to either normoxia (NMX, 21%O2) or HPX (10.5%O2) starting at 28-day (early onset, EO-HPX) or 50-day (late onset, LO-HPX) gestation until term (65 days). Near-term male and female fetuses were extracted from anesthetized sows, and mitochondria were isolated from excised fetal forebrains (n = 6/group). Expression of mitochondrial complex subunits I–V (CI–CV), fission (Drp-1), and fusion (Mfn-2) proteins was measured by Western blot. CI and CIV enzyme activities were measured by colorimetric assays. Chronic HPX reduced fetal body wts and increased (P |
| Databáze: | OpenAIRE |
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