In Vitro Characterization of Lactic Acid Bacteria Isolated from Bovine Milk as Potential Probiotic Strains to Prevent Bovine Mastitis
| Autor: | Cristina Bogni, Ignacio D Frola, M Pellegrino, Dino Gobelli, Berardo Natanael, María Elena Nader-Macías |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
030106 microbiology Microbiology Bacterial Adhesion law.invention BOVINE MASTITIS 03 medical and health sciences Probiotic chemistry.chemical_compound Anti-Infective Agents Lactobacillales LACTIC ACID BACTERIA law medicine Pulsed-field gel electrophoresis Animals Mastitis Bovine Molecular Biology biology Probiotics Otras Ciencias Químicas PROBIOTIC Lactococcus lactis Ciencias Químicas Epithelial Cells Antimicrobial biology.organism_classification medicine.disease PREVENTION In vitro Lactic acid Mastitis Milk 030104 developmental biology chemistry Molecular Medicine Cattle Female CIENCIAS NATURALES Y EXACTAS Bacteria |
| Zdroj: | Probiotics and Antimicrobial Proteins. 11:74-84 |
| ISSN: | 1867-1314 1867-1306 |
| DOI: | 10.1007/s12602-017-9383-6 |
| Popis: | Bovine mastitis causes economic losses on dairy farms worldwide. Lactic acid bacteria (LAB) in animal health are an alternative tool to avoid antibiotic therapy on the prevention of bovine mastitis. In previous studies, 12 LAB isolated from bovine milk were selected taking into account some of the following characteristics: hydrophobicity, auto aggregative capability, inhibition of indicator pathogens, hydrogen peroxide, and capsular polysaccharide production. These LAB were considered because of their beneficial properties. In this work, we also analyzed the antimicrobial activity and the co-aggregation against mastitis causing bacteria, auto-inhibition, adhesion to bovine teat canal epithelial cells (BTCEC), and growth kinetic curves for the 12 LAB. Two of them, Lactococcus lactis subsp. lactis CRL 1655 and Lactobacillus perolens CRL 1724, were selected because they had an interesting pattern of adhesion to BTEC, the inhibition of pathogens and the co-aggregation with the 100% of the assayed pathogens. They showed a predictable difference in the PFGE genomic pattern bands. The kinetic growth of these two strains was similar between them and with the rest of the assayed LAB. The strains selected in the present study showed indispensable characteristics for their inclusion in a probiotic formulation to be used at dry-off period for the prevention of bovine mastitis. Fil: Pellegrino, Matias Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina Fil: Frola, Ignacio Daniel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina Fil: Berardo, Natanael. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina Fil: Gobelli, Dino Joaquin. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Bogni, Cristina Ines. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina |
| Databáze: | OpenAIRE |
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