Class I TCP proteins TCP14 and TCP15 are required for elongation and gene expression responses to auxin
| Autor: | Daniel H. Gonzalez, Federico Ariel, Lucia Ferrero, Victoria Gastaldi, Ivana L. Viola |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Hot Temperature Mutant Arabidopsis Plant Science 01 natural sciences 03 medical and health sciences Gene Expression Regulation Plant Auxin Gene expression Genetics Arabidopsis thaliana heterocyclic compounds Gene Transcription factor chemistry.chemical_classification Indoleacetic Acids biology Arabidopsis Proteins fungi food and beverages RNA Promoter General Medicine biology.organism_classification Chromatin Hypocotyl Cell biology 030104 developmental biology chemistry Agronomy and Crop Science Transcription Factors 010606 plant biology & botany |
| Zdroj: | Plant Molecular Biology. 105:147-159 |
| ISSN: | 1573-5028 0167-4412 |
| Popis: | Two class I TCP transcription factors are required for an efficient elongation of hypocotyls in response to auxin and for the correct expression of a subset of auxin-inducible genes In this work, we analyzed the response to auxin of plants with altered function of the class I TEOSINTE BRANCHED 1, CYCLOIDEA, PCF (TCP) transcription factors TCP14 and TCP15. Several SMALL AUXIN UP RNA (SAUR) genes showed decreased expression in mutant plants defective in these TCPs after an increase in ambient temperature to 29 °C, a condition that causes an increase in endogenous auxin levels. Overexpression of SAUR63 caused a more pronounced elongation response in the mutant than in the wild-type at 29 °C, suggesting that the decreased expression of SAUR genes is partly responsible for the defective elongation at warm temperature. Notably, several SAUR genes and the auxin response gene IAA19 also showed reduced expression in the mutant after auxin treatment, while the expression of other SAUR genes and of IAA29 was not affected or was even higher. Expression of the auxin reporter DR5::GUS was also higher in a tcp15 mutant than in a wild-type background after auxin treatment. However, the elongation of hypocotyls in response to auxin was impaired in the mutant. Remarkably, a significant proportion of auxin inducible genes and of targets of the AUXIN RESPONSE FACTOR 6 are regulated by TCP15 and often contain putative TCP recognition motifs in their promoters. Furthermore, we demonstrated that several among them are recognized by TCP15 in vivo. Our results indicate that TCP14 and TCP15 are required for an efficient elongation response to auxin, most likely by regulating a subset of auxin inducible genes related to cell expansion. |
| Databáze: | OpenAIRE |
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