Efficient isolation of Swine influenza viruses by age-targeted specimen collection
| Autor: | Toshiko Kawabata, Eisaburo Deguchi, Makoto Ozawa, Manabu Igarashi, Aya Matsuu, Kouki Yonezawa, Akira Taneno, Kyoko Tsukiyama-Kohara, Kosuke Okuya, Kimihito Ito |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Microbiology (medical)
Genotype Swine viruses animal diseases Molecular Sequence Data Population Sequence Homology Hemagglutinin (influenza) Hemagglutinin Glycoproteins Influenza Virus Virus Clinical Veterinary Microbiology Specimen Handling Microbiology Orthomyxoviridae Infections Neutralization Tests Seroepidemiologic Studies Pandemic Animals Humans Seroprevalence Microneutralization Assay education Phylogeny Swine Diseases education.field_of_study biology Age Factors virus diseases Outbreak Sequence Analysis DNA Orthomyxoviridae Virology Specimen collection Epidemiological Monitoring biology.protein Nasal Cavity |
| Zdroj: | Journal of Clinical Microbiology |
| ISSN: | 0095-1137 |
| Popis: | The control of swine influenza virus (SIV) infection is paramount for increasing the productivity of pig farming and minimizing the threat of pandemic outbreaks. Thus, SIV surveillance should be conducted by region and on a regular basis. Here, we established a microneutralization assay specific for SIV seroprevalence surveillance by using reporter gene-expressing recombinant influenza viruses. Growth-based SIV seroprevalence revealed that most sows and piglets were positive for neutralizing antibodies against influenza viruses. In contrast, the 90-day-old growing pigs exhibited limited neutralizing activity in their sera, suggesting that this particular age of population is most susceptible to SIV infection and thus is an ideal age group for SIV isolation. From nasal swab specimens of healthy pigs in this age population, we were able to isolate SIVs at a higher incidence (5.3%) than those of previous reports. Nucleotide sequencing and phylogenetic analysis of the hemagglutinin (HA) genes revealed that the isolated SIVs have circulated and evolved in pigs but not have been recently introduced from humans, implying that a large number of SIV lineages may remain “undiscovered” in the global porcine populations. We propose that the 90-day-old growing pig-targeted nasal swab collection presented in this study facilitates global SIV surveillance and contributes to the detection and control of SIV infection. |
| Databáze: | OpenAIRE |
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